, Volume 9, Issue 1, pp 81–90 | Cite as

Influence of fluid-phase chemoattractants on polymorphonuclear leukocyte chemotaxic responsiveness to a surface-bound attractant

  • Claes Dahlgren
Original Articles


Polymoiphonuclear leukocytes (PMNLs) were allowed to migrate on slides with fixed yeast particles dotted about on the surface. Locomotion was quantified by counting the number of yeast particles in association with a PMNL, Addition of a complement source to yeast particles able to activate the complement system resulted in a chemotactic response even when fluid-phase attractants were removed prior to the measurement of PMNL chemotaxis, indicating that surface-bound attractants guided the PMNLs to the yeast particles. The presence of high concentrations of fluid-phase chemoattractants resulted in a reduced PMNL chemotactic response to the surface-bound gradient. From comparisons between the yeast-slide system and the locomotion-under-agarose assay, it could be concluded that PMNL chemotaxis in response to a surface-bound gradient is less influenced by factor-specific deactivation than the response to a fluid-phase attractant. The PMNL chemotactic response is reduced to both surface-bound and fluid-phase gradients as a result of a non-factorspecific deactivation.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Klebanoff, S. J., andR. A. Clark. 1978. The Neutrophil. North-Holland Publishing Co., New York.Google Scholar
  2. 2.
    Dahlgren, C. 1982. Locomotion and adhesion of polymorphonuclear leukocytes.Cell Biophys. 4:133–141.Google Scholar
  3. 3.
    Dierich, N. P., W. Sablotny, andG. Till. 1980. Migration of leukocytes into filters coated homogeneously with immune complexes, antigens, lectins or tripeptides.Imtnunobiology 157:47–53.Google Scholar
  4. 4.
    Repo, H. 1976. Leukocyte migration agarose test for the assessment of human neutrophil chemotaxis. Thesis, University of Helsinki, Finland.Google Scholar
  5. 5.
    Dahlqren, C., andH. Elwing. 1983. Inhibition of polymorphonuclear leukocyte locomotion by surface bound antigen-antibody complexes.Immunology 49:329–336.Google Scholar
  6. 6.
    Wilkinson, P. C. 1982. Chemotaxis and Inflammation, 2nd ed. Churchill Livingston, Edinburgh.Google Scholar
  7. 7.
    Dahlgren, C., J. Hed, andO. Stendahl. 1984. Chemotaxis of polymorphonuclear leukocytes in response to surface-bound complement, derived chemoattractants generated in situ.Inflammation 8:201–208.Google Scholar
  8. 8.
    Webster, R. O., B. Sanolari, andP. M. Hensox. 1980. Neutrophil Chemotaxis in response to surface bound C5a.Exp. Cell Res. 129:55–62.Google Scholar
  9. 9.
    Wilkinson, P. C., andR. B. Allan. 1978. Chemotaxis of neutrophil leukocytes towards substratum-bound protein attractants.Exp. Cell Res. 117:403–412.Google Scholar
  10. 10.
    Issekutz, A. C., andW. D. Biggar. 1977. Influence of serumderived chemotactic factors and bacterial products on human neutrophil chemotaxis.Infect. Immun. 15:212–220.Google Scholar
  11. 11.
    Ward, P. A., andE. L. Becker. 1968. The deactivation of rabbit neutrophils by chemotactic factor and the nature of the activatible esterase.J. Exp. Med. 127:693–709.Google Scholar
  12. 12.
    Van Epps, D. E., andB. R. Andersen. 1973. Streptolysin 0 inhibition of neutrophil chemotaxis and mobility: Nonimmune phenomenon with species specificity.Infect. Immun. 9:27–33.Google Scholar
  13. 13.
    Goetzl, E. J., I. Gigli, S. Wasserman, andK. F. Austen. 1973. A neutrophil immobilizing factor derived from human leukocytes. II. Specificity of action on polymorphonuclear leukocyte mobility.J. Immunol. 111:938–945.Google Scholar
  14. 14.
    Nelson, R.D., R. T. McCormack, C. D. Fiegel, andR. L. Simmons. 1978. Chemotactic deactivation of human neutrophils: Evidence for nonspecific and specific components.Infect. Immun. 22:441–444.Google Scholar
  15. 15.
    Nelson, R. D., V. D. Fiegel, J. Herron, J. M. Gracyk, M. P. Bauman, R. T. McCormack, andR. L. Simmons. 1980. Chemotactic deactivation of human neutrophils: Role of stimulation of hexosemonophosphate shunt activity in nonspecific deactivation.Acta Physiol. Scand, Suppl. 492:31–41.Google Scholar
  16. 16.
    Coble, B. I., C. Dahlgren, J. Hed, andO. Stendahl. 1983. A quantitative microassay for leukocyte chemotaxis, using a microscopic slide system with complement-activation yeast particles as gradient source.J. Immunol. Methods 64:303–311.Google Scholar
  17. 17.
    Bøyum, A. 1968. Isolation of mononuclear cells and granulocytes from human blood.Scand. J. Clin. Lab. Invest. Suppl. 97:77–89.Google Scholar
  18. 18.
    Stendahl, O., L. Molin, andC. Dahlgren. 1978. The inhibition of polymorphonuclear leukocyte cytotoxicity by dapsone.J. Clin. Invest. 62:214–220.Google Scholar
  19. 19.
    Sullivan, S. J., andS. H. Zigmond. 1980. Chemotactic peptide receptor modulation in polymorphonuclear leukocytes.J. Cell Biol 85:703–711.Google Scholar
  20. 20.
    Zigmond, S. H., andS. J. Sullivan. 1979. Sensory adaption of leukocytes to Chemotactic peptidesJ. Cell. Biol 82:517–527.Google Scholar

Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Claes Dahlgren
    • 1
  1. 1.Department of Medical MicrobiologyUniversity of Linköping School of MedicineLinköpingSweden

Personalised recommendations