Journal of Clinical Immunology

, Volume 6, Issue 1, pp 65–73 | Cite as

The role of receptors for complement in the induction of polyclonal B-cell proliferation and differentiation

  • Thomas F. Tedder
  • Janis J. Weis
  • Loran T. Clement
  • Douglas T. Fearon
  • Max D. Cooper
Original Articles
Accepted:

Abstract

A panel of monoclonal antibodies and ligands that bind to the CR1 or CR2 complement receptors of B cells has been used to investigate the role of these membrane molecules in regulating B-cell proliferation and differentiation. When CR2 was modulated from the surface of B cells by treatment with the HB-5 antibody and a secondary goat anti-mouse immunoglobulin antibody, Epstein-Barr virus-induced polyclonal B-cell proliferation and immunoglobulin production were inhibited by 83 and 90%, respectively. In contrast, modulation of other cell surface molecules, HB-2, B1, and the C3b receptor (CR1), or pretreatment of B cells with C3d,g (a CR2 ligand) or HB-5 antibody, alone minimally inhibited these responses. Neither the HB-5 antibody C3d,g, nor a monoclonal antibody (YZ-1) reactive with CR1 induced resting B cells to proliferate, nor did they alter anti-μ antibody-induced proliferation. Similarly, treatment with C3d,g or with the HB-5 or YZ-1 antibodies did not induce B cells to secrete immunoglobulin or affect pokeweed mitogen-induced plasma-cell formation. Whereas CR2 appears to be the functionally relevant receptor for Epstein-Barr virus on B cells, the effects of ligand interactions with CR1 and CR2 on normal B-cell proliferation or differentiation remain unidentified.

Key words

Complement receptors B cells Epstein-Barr virus 
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Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • Thomas F. Tedder
    • 1
  • Janis J. Weis
    • 2
  • Loran T. Clement
    • 1
  • Douglas T. Fearon
    • 2
  • Max D. Cooper
    • 1
  1. 1.The Cellular Immunobiology Unit of the Tumor Institute, Departments of Pediatrics and Microbiology, and The Comprehensive Cancer CenterUniversity of Alabama in BirminghamBirmingham
  2. 2.Department of Medicine, Harvard Medical School, and Department of Rheumatology and ImmunologyBrigham and Women's HospitalBoston

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