Sixteen patients with ragweed pollenosis were studied immediately before and after the 1979 ragweed season with measurement of total plasma IgE, specific plasma IgE against ragweed antigen E (IgE-a-AgE), molecules of IgE-a-AgE eluted per basophil, total antibody binding of AgE (blocking antibody), and maximum percentage histamine release in response to AgE. Thirteen received immunotherapy with polymerized ragweed and the measurements were repeated just before and after 1980 ragweed season. The patients kept symptom score diaries for each ragweed season. With season before immunotherapy, plasma IgE-a-AgE and total IgE rose. The ratio of specific to total IgE has been demonstrated to correlate with the IgE-a-AgE molecules eluted per basophil. This ratio, molecules of IgE-a-AgE eluted per basophil, and histamine release did not change. With immunotherapy, there was a significant decrease in symptom scores and a marked increase in blocking antibody. Specific IgE in plasma remained the same as total IgE fell from October 1979 to July 1980. Thus, there was a modest increase in ratio and in the number of IgE-a-AgE molecules eluted per basophil. With these changes, there was no alteration of histamine release. With season, after immunotherapy, there was not significant change in any parameter measured.
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Yunginger JW, Gleich GJ: Seasonal changes in IgE antibodies and their relationship to IgG antibodies during immunotherapy for ragweed hayfever. J Clin Invest 52:1268–1275, 1973
Gleich GJ, Jacobs GL, Yunginger JW, Henderson LS: Measurement of the absolute levels of IgE antibodies in patients with ragweed hayfever. J Allergy Clin Immunol 60:188–198, 1978
Yunginger JW, Gleich GJ: Seasonal change in serum and nasal IgE concentrations. J Allergy Clin Immunol 51:174–186, 1973
Berg T, Johansson SGO: IgE concentrations in children with atopic diseases. Int Arch Allergy Appl Immunol 36:219–232, 1969
Levy DA, Lichtenstein LM, Goldenstein EO, Ishizaka K: Immunologic and cellular changes accompanying the therapy of pollen allergy. J Clin Invest 50:360–369, 1971
Irons JS, Pruzansky JJ, Patterson R, Zeiss CR: Studies of perennial ragweed immunotherapy. J Allergy Clin Immunol 59:190–199, 1977
Cook RA, Barnard JH, Hebald S, Stull A: Serologic evidence of immunity with coexisting sensitization in a type of human allergy. J Exp Med 62:733–750, 1935
Zeiss CR, Metzger WJ, Levitz D: Quantitative relationships between IgE antibody and blocking antibodies specific for antigen E in patients given immunotherapy with ragweed antigen E. Clin Exp Immunol 28:250–255, 1977
Pruzansky JJ, Patterson R: Histamine release from leukocytes of hypersensitive patients. II. Reduced sensitivity of leukocytes after injection therapy. J Allergy 39:44–50, 1967
Rocklin RE, Sheffer AL, Greineder DK, Melmon KL: Generation of antigen-specific suppressor cells during allergy desensitization. N Engl J Med 302:1213–1219, 1980
Ishizaka K, Soto CS, Ishizaka T: Mechanisms of passive sensitization. III. Number of IgE molecules and their receptor sites on human basophil granulocytes. J Immunol 111:500–511, 1973
Ishizaka T, Ishizaka K: Mechanisms of passive sensitization. IV. Dissociation of IgE molecules from basophil receptor at acid pH. J Immunol 112:1078–1084, 1974
Conroy MC, Adkinson NF, Sobotka AK, Lichtenstein LM: Measurement of IgE on human basophils: Relation to serum IgE and anti-IgE induced histamine release. J Immunol 118:1317–1321, 1977
Zeiss CR, Pruzansky JJ, Levitz D, Wang J: The quantification of IgE antibody specific for ragweed antigen E from the basophil surface in patients with ragweed pollenosis. Immunology 35:237–246, 1978
Hendrix SG, Patterson R, Zeiss CR, Pruzansky JJ, Suszko IM, McQueen RC, Slavin RG, Miller MP, Lieberman PL, Sheffer AL: A multi-institutional trial of polymerized whole ragweed for immunotherapy of ragweed allergy. J Allergy Clin Immunol 66:486–494, 1980
Gilbert HS, Ornstein L: Basophil counting with a new staining method using alcian blue. Blood 46:279–286, 1975
King TP, Norman PS, Connell JT: Isolation and characterization of allergens from ragweed pollen. II. Biochemistry 3:458–468, 1964
Ishizaka K, Ishizaka T, Lee FH: Physiochemical properties of reaginic antibody. J Allergy 37:336–349, 1966
Zeiss CR, Kanellakes T, Bellone J, Levitz D, Pruzansky JJ, Patterson R: IgE-mediated asthma and hypersensitivity pneumonitis with precipitating anti-hapten antibodies due to MDI exposure. J Allergy Clin Immunol 65:346–352, 1980
Pruzansky JJ, Patterson R: Decrease in basophils after incubation with specific antigens of leukocytes from allergic donors. Int Arch Allergy Appl Immunol 38:522–526, 1970
Zeiss CR, Pruzansky JJ, Patterson R, Roberts M: A solid phase radioimmunoassay for the quantitation of human reaginic antibody against ragweed antigen E. J Immunol 110:414–421, 1973
Lidd, D, Farr RS: Primary interaction between131I labeled ragweed pollen and antibodies in the sera of humans and rabbits. J Allergy 33:45–58, 1962
Ishizaka K, Tomioka H, Ishizaka T: Mechanisms of passive sensitization. I. Presence of IgE and IgG molecules on human leukocytes. J Immunol 105:1459–1467, 1970
Stallman PJ, Aalberse RC: Estimation of basophil-bound IgE by quantitative immunofluorescence microscopy. Int Arch Allergy Appl Immunol 54:9–18, 1977
Spiegelberg HL, O'Connor RD, Simon RA, Mathison DA: Lymphocytes with immunoglobulin E Fc receptors in patients with atopic disorders. J Clin Invest 64:714–720, 1979
Malveaux FL, Conroy MC, Adkinson NF, Lichtenstein LM: IgE receptors on human basophils: Relationship to serum IgE concentration. J Clin Invest 62:176–181, 1978
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Grammer, L.C., Zeiss, C.R., Levitz, D. et al. Variation with season and with polymerized ragweed immunotherapy in IgE against ragweed antigen E in plasma and eluted from the basophil surface in patients with ragweed pollenosis. J Clin Immunol 1, 222–227 (1981). https://doi.org/10.1007/BF00915140
- Basophil-bound IgE
- IgE against ragweed antigen E