Human polymorphonuclear leukocytes (PMNL) can metabolize [l-14C]2-deoxyglucose to14CO2 when stimulated by either phorbol myristate acetate (PMA) or opsonized zymosan. Oxidation of 2-deoxyglucose is about 10–20% as efficient as that of glucose in normal PMNL; it does not occur in defective cells obtained from patients with chronic granulomatous disease. The increased oxidation of [ l-14C]2-deoxyglucose induced by PMA is not sufficient to explain the inhibition of transport induced by that compound; conversely increased transport of 2-deoxyglucose induced by zymosan-activated serum does not result in a significant increase in oxidation of the hexose. Oxidation of [l-14C]2-deoxyglucose appears to be mediated by the hexose monophosphate shunt as indicated by the following (1) oxidation of [1-14C]2-deoxyglucose in intermediate in activity between that of [l-14C]glucose and [6-14C]glucose; (2) the reaction is insensitive to cyanide or azide; and (3) shunt enzymes measured in a cell-free extract from human PMNL can react with 2-deoxyglucose compounds with approximately 10% the efficiency shown towards the corresponding glucose derivatives.
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DeChatelet, L. R. 1978. Initiation of the respiratory burst in human polymorphonuclear neutrophils: A critical review.J. Reticuloendothel. Soc. 24:73–91.
DeChatelet, L. R., andM. R. Cooper. 1970. A modified procedure for the determination of leukocyte alkaline phosphatase.Biochem. Med. 4:61–68.
DeChatelet, L. R., P. S. Shirley, andR. B. Johnson, Jr. 1976. Effect of phorbol myristate acetate on the oxidative metabolism of human polymorphonuclear leukocytes.Blood.47:545–554.
Gee, J. B. L., A. S. Khandwala, andR. W. Bell. 1974. Hexose transport in the alveolar macrophage: Kinetics and pharmacologie features.J. Reticuloendothel. Soc. 15:394–405.
Holmes, B., A. R. Page, andR. A. Good. 1967. Studies of the metabolic activity of leukocytes from patients with a genetic abnormality of phagocytic function.J. Clin. Invest. 46:1422–1432.
Horecker, B. L., andP. Z. Smyrniotis. 1955. 6-Phosphogluconic dehydrogenase,In Methods in Enzymology, Vol. 1, S. P. Colowick and N. O. Kaplan, editors. Academic Press Inc., New York. 326–327.
Kornberg, A., andB. L. Horecker. 1955. Glucose-6-phosphate dehydrogenase,In Methods in Enzymology, Vol. 1. S. P. Colowick and N. O. Kaplan, editors. Academic Press Inc., New York. 323–326.
Lowry, O. H., N. J. Rosebrough, A. L. Farr, andR. J. Randall. 1951. Protein measurement with the Folin phenol reagent.J. Biol Chem. 193:265–275.
Mukkada, A. J., andP. F. Bonventre. 1975. Membrane transport by mouse and guinea pig macrophages: characteristics of the glucose transport system.J. Reticuloendothel. Soc. 17:20–29.
Olefsky, J. M., andM. Kobayashi. 1978. Ability of circulating insulin to chronically regulate the cellular glucose transport system.Metabolism 27:1917–1928.
Sanders, D. Y., M. R. Cooper, C. E. McCall, andL. R. Dechatelet. 1972. Chronic granulomatous disease of childhood with onset of symptoms at age 11 years.J. Pediatr. 80:104–106.
Zabos, P., D. Kyner, N. Mendelsohn, C. Schreiber, S. Waxman, J. Christman, and G. Acs. 1978. Catabolism of 2-deoxyglucose by phagocytic leukocytes in the presence of 12-0-tetradecanoyl phorbol-13-acetate.Proc. Natl. Acad. Sci. U.S.A. 75:5422–5426.
McCall, C. E., D. A. Bass, L. R. Dechatelet, andM. S. Cousart. 1979. C5a has insulinlike activity for neutrophils. Abstr.,Clin. Res. 27:472A.
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Dechatelet, L.R., Campbell, T.L., McCall, C.E. et al. Oxidation of 2-deoxyglucose by human polymorphonuclear leukocytes. Inflammation 4, 249–259 (1980). https://doi.org/10.1007/BF00915026
- Phorbol Myristate Acetate