, Volume 13, Issue 4, pp 443–453 | Cite as

Effects of products from inflammatory pulmonary neutrophils on alveolar macrophage chemotaxis, spreading, and thymidine incorporation

  • K. Donaldson
  • J. Slight
  • D. M. Brown
Original Articles


Since macrophages and neutrophils are found together in the alveolar region of the lung during alveolar inflammation, we assessed whether neutrophil products could influence three key functions of alveolar macrophages: chemotaxis, spreading, and thymidine incorporation. Neutrophils were obtained from the lungs of rats treated by intratracheal instillation of heat-killedCorynebacterium parvum and cultured overnight, alone or in the presence of zymosan, PMA, an inert particulate (titanium dioxide), or a toxic dust, (quartz). Supernatants were collected from these cells and a lysate, obtained by freeze-thawing neutrophils, was also used. Neutrophil supernatants caused a slight reduction in chemotaxis and a significant loss of ability to spread on glass which varied depending on the in vitro treatment of the neutrophils. In addition neutrophil supernatants also had a substantial effect in stimulating uptake of thymidine which was, once again, very dependent on the treatment of the neutrophils during preparation of the supernatants, with unstimulated and TiO2-treated neutrophils producing maximum stimulation. The increases in thymidine uptake were not matched by increased proliferation, suggesting that another signal may be necessary for expansion of alveolar macrophage numbers during alveolar inflammation.


Titanium Thymidine Alveolar Macrophage Substantial Effect Slight Reduction 
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  1. 1.
    Keogh, B. A., and R. G.Crystal. Alveolitis-the key to the interstitial lung disorders.Thorax 37:1–10.Google Scholar
  2. 2.
    Crystal, R. G., J. E. Gadek, V. J. Ferrans, J. D. Fulmer, B. R. Line, andG. W. HunNinghake. 1981. Interstitial lung disease: current concepts of pathogenesis, staging and therapy.Am. J. Med. 70:542–568.Google Scholar
  3. 3.
    Young, K. R., andH. Y. Reynolds. 1984. Bronchoalveolar washings: proteins and cells from normal lungs.In Immunology of the Lung and Upper Respiratory Tract. J. Bienenstock, editor. McGraw-Hill, New York. 157–173.Google Scholar
  4. 4.
    Gellert, A. R., J. A. Langford, S. Uthayakumar, andR. M. Rudd. 1985. Bronchoalveolar lavage and clearance of99mTc-DTPA in asbestos workers without evidence of asbestosis.Br. J. Dis. Chest. 79:251–257.Google Scholar
  5. 5.
    Weiland, J. E., W. B. Davis, J. F. Holter, J. R. Mohammed, P. M. Dorinsky, andJ. E. Gadek. 1986. Lung neutrophils in the adult respiratory distress syndrome.Am. Rev. Respir. Dis. 133:218–225.Google Scholar
  6. 6.
    Wright, D. G. 1982. The neutrophil as a secretory organ of host defence.In Advances in Host Defense Mechanisms, Vol. 1. J. I. Gallin and A. S. Fausi, editors. Raven Press, New York. 75–110.Google Scholar
  7. 7.
    Yoshinaga, M., S. Nakamura, andH. Hayashi. 1975. Interaction between lymphocytes and inflammatory exudate cells I Enhancement of thymocyte response to PHA by product(s) of polymorphonuclear leukocytes and macrophages.J. Immunol. 115:533–537.Google Scholar
  8. 8.
    Lamster, I. B., S. T. Sonis, D. M. Mirando, andR. E. Wilson. 1980. Influence of supernatants from polymorphonuclear leukocytes on blastogenesis of syngeneic and allogeneic murine splenocytes.Clin. Exp. Immunol. 36:285–291.Google Scholar
  9. 9.
    Ishibashi, Y., andT. Yamashita. 1987. Effects of a phagocytosis-stimulatory factor derived from polymorphonuclear neutrophils on the functions of macrophages.Infect. Immun. 55:1762–1766.Google Scholar
  10. 10.
    Nakamura, S., F. Goto, K. Goto, andM. Yoshinaga. 1982. Physiochemical characterisation of a PMN-derived soluble factor that enhances lymphocyte DNA synthesis.J. Immunol. 128:2614–2621.Google Scholar
  11. 11.
    Bird, J., V. J. Sheng, andJ. P. Giroud. 1984. Effects of supematants and lysates of polymorphonuclear leukocytes: macrophage stimulatory factors.Br. J. Exp. Pathol. 65:243–250.Google Scholar
  12. 12.
    Hunninghake, G. W., J. E. Gadek, I. Kawanami, J. Ferrans, andR. G. Crystal. 1979. Inflammatory and immune processes in the human lung in health and disease. Evaluation by bronchoalveolar lavage.Am. J. Pathol. 97:149–206.Google Scholar
  13. 13.
    Donaldson, K., R. E. Bolton, D. M. Brown, andA. Douglas. 1984. An improved macrophage spreading assay — a simple and effective measure of activation.Immunol. Commun. 13:229–244.Google Scholar
  14. 14.
    Donaldson, K., J. Slight, andR. E. Bolton. 1988. The effect of products from bronchoalveolar derived neutrophils on oxidant production and phagocytic activity of alveolar macrophages.Clin. Exp. Immunol. 74:477–482.Google Scholar
  15. 15.
    Donaldson, K., R. E.Bolton, D. M.Brown, G. M.Brown, H.Cowie, A. D.Jones, M. D.Robertson, J.Slight, and J. M. G.Davis. 1988. Studies on the cellular response in lung tissue to the inhalation of mineral dust. Institute of Occupational Medicine Report No. TM/88/01.Google Scholar
  16. 16.
    Wynne, K. M., W. G. Spector, andD. A. Willoughby. 1975. Macrophage proliferation in vitro induced by exudates.Nature 253:636–637.Google Scholar
  17. 17.
    Adolphe, M., J. Fontagne, M. Pelletier, J. P. Giroud. 1975. Induction of DNA synthesis in rat macrophages in vitro by inflammatory exudates.Nature 253:636.Google Scholar
  18. 18.
    Dientsman, S. R., andV. Defendi. 1978. Necessary and sufficient conditions for recruitment of macrophages into the cell cycle.Exp. Cell Res. 115:191–199.Google Scholar
  19. 19.
    Hamilton, J. A., andS. R. Dientsman. 1981. Induction of macrophage DNA synthesis by phorbol esters.J. Cell. Physiol. 106:445–450.Google Scholar
  20. 20.
    Richards, R. J., N. Davies, J. Atkins, V. I. C. Oreffo. 1987. Isolation, biochemical characterization and culture of lung type II cells of the rat.Lung 165:143–158.Google Scholar

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • K. Donaldson
    • 1
  • J. Slight
    • 1
  • D. M. Brown
    • 1
  1. 1.Institute of Occupational MedicineEdinburghScotland

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