The hamster cheek pouch microcirculation was used to investigate the effects of platelet-activating factor (PAF) on leukocyte adhesion to microvascular walls by means of intravital microscopy. PAF was applied topically at concentrations ranging from 10−11 to 10−5 M. An inverse relationship between PAF concentration and number of adhering white cells per 100-Μm length was found in venules ranging in diameter from 10 to 60 Μm (grouped into 10-Μm intervals). Importantly, the PAF-induced adhesion of leukocytes lasted for the 3-h experimental period. We postulate that induction of leukocyte adhesion to venular endothelium is an important role of PAF in inflammatory processes.
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Pinckard, R. N., L. M. McManus, andD. J. Hanahan. 1982. Chemistry and biology of acetyl glyceryl ether phosphorylcholine (platelet-activating factor).In Advances in Inflammation Research, Vol. 4. G. Weissmann, editor. Raven Press, New York. 147–180.
O'Flaherty, J. 1982. Biology of disease lipid mediators of inflammation and allergy.Lab Invest. 47:314–328.
Pinckard, R. N. 1983. Platelet-activating factor.Hosp. Proc. 18(11):67–76.
Clark, P. O., D. J. Hanahan, andR. N. Pinckard. 1980. Physical and chemical properties of platelet-activating factor obtained from human neutrophils and monocytes and rabbit neutrophils and basophils.Biochim. Biophys. Acta 628:69–75.
Arnoux, B., D. Duval, andJ. Benveniste. 1980. Release of platelet-activating factor (PAF-acethes) from alveolar macrophages by the calcium ionophore A23187 and phagocytosis.Eur. J. Clin. Invest. 10:437.
Camussi, G., M. Aglietta, R. Coda, andF. Bussolino. 1981. Release of platelet-activating factor and histamine. II. The cellular origin of human PAF: Monocytes, polymorphonuclear neutrophils and basophils.Immunology 42:191–199.
Camussi, G., M. Aglietta, F. Malavasi, A. Tetta, W. Piacibello, F. Sanavio, andF. Bussolino. 1983. The release of platelet-activating factor from human endothelial cells in culture.J. Immunol. 131:2397–2403.
O'Flaherty, J. T., R. L. Wykle, C. H. Miller, J. C. Lewis, M. Waite, D. A. Bars, C. E. McCall, andL. R. Dechatelet. 1981. 1-O-alkyl-sn-glyceryl-3-phosphorylcholines. A novel class of neutrophil stimulants.Am. J. Pathol. 103:70–78.
Shaw, J. O., R. N. Pinckard, K. S. Ferrioini, L. M. McManus, D. J. Hanahan. 1981. Activation of human neutrophils with 1-O-hexadecyl octadecyl-2-acetyl-sn-glyceryl-3 phosphorylcholine (platelet activating factor).J. Immunol. 127:1250–1255.
Ingraham, L., T. Coates, J. Allen, C. Higgins, R. Baehner, andL. Boxer. 1982. Metabolic, membrane, and functional responses of human polymorphonuclear leukocytes to platelet-activating factor.Blood 59:1259–1266.
Gimbrone, M. A., A. F. Brock, andA. L. Schafer. 1984. Leukotriene B4 stimulates polymorphonuclear leukocyte adhesion to cultured vascular endothelial cells.J. Clin. Invest. 74:1552–1555.
Bjork, J., L. Lindbom, B. Gerdin, G. Smedegard, K. E. Arfors, andJ. Benveniste. 1983. PAF-acether (platelet activating factor) increases microvascular permeability and affects endothelium-granulocyte interaction in microvascular beds.Acta Physiol. Scand. 119:305–308.
McManus, L. M., D. J. Hanahan, C. A. Demopoulos, andR. N. Pinckard. 1980. Pathobiology of the intravenous infusion of acetyl glyceryl ether phosphorylcholine (AGEPC), a synthetic platelet-activating factor (PAF) in the rabbit.J. Immunol. 124:2919–2924.
McManus, L., N. Pinckard, F. Fitzpatrick, R. O'Rourke, M. Crawford, andD. Hanahan. 1981. Acetyl glyceryl ether phosphorylcholine intravascular alterations following intravenous infusion into the baboon.Lab. Invest. 45:303–307.
Archer, C. B., C. P. Page, J. Morley, D. M. MacDonald. 1985. Accumulation of inflammatory cells in response to intracutaneous platelet activating factor (PAF-acether) in man.Br. J. Dermatol. 112:285–290.
Humphrey, D. M., D. J. Hanahan, andR. N. Pinckard. 1982. Induction of leukocyte infiltrates in rabbit skin by acetyl glyceryl ether phosphorylcholine.Lab. Invest. 47:227–234.
Mayhan, W. G., andW. L. Joyner. 1984. The effect of altering the external calcium concentration and a calcium channel blocker, verapamil, on microvascular leaky sites and dextran clearance in the hamster cheek pouch.Microvasc. Res. 28:143–158.
Atherton, A., andG. V. R. Born. 1972. Quantitative investigations of the adhesiveness of circulating polymorphonuclear leukocytes to blood vessel walls.J. Physiol. 222:447–474.
Mayrovitz, H. N., S. J. Kang, B. Herscovici, andR. N. Sampsell. 1987. Leukocyte adherence initiation in skeletal muscle capillaries and venules.Microvasc. Res. 33:22–34.
Dillon, P. K., andW. N. Durán. 1988. The effect of platelet-activating factor on microvascular permselectivity: Dose-response relationships and pathways of action in the hamster cheek pouch microcirculation.Circ. Res. 62:732–740.
Dillon, P. K., A. B. Ritter, andW. N. Durán. 1988. Vasoconstrictor effects of plateletactivating factor in the hamster cheek pouch microcirculation: Dose-related relationships and pathways of action.Circ. Res. 62:722–731.
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Dillon, P.K., Fitzpatrick, M.F., Ritter, A.B. et al. Effect of platelet-activating factor on leukocyte adhesion to microvascular endothelium. Inflammation 12, 563–573 (1988). https://doi.org/10.1007/BF00914318
- Public Health
- Internal Medicine
- Inflammatory Process
- Inverse Relationship
- Experimental Period