Inflammation

, Volume 14, Issue 5, pp 561–569 | Cite as

Characterization of leukocyte chemotactic activity of bacteriocin fromStreptococcus mutans Rm-10

  • Yoshio Tsukamoto
  • Sachiko Fukutani
  • Yasuyuki Takayama
  • Hisanori Fukushima
  • Hirosuke Sagawa
  • Masakazu Mori
Original Articles

Abstract

Bacteriocins have several biological activities in addition to their antibacterial effect. We investigated the chemotactic properties and mode of action of purified streptococcal bacteriocin. Bacteriocin purified from a culture supernatant ofStreptococcus mutans (S. mutans) Rm-10 induced chemotaxis of human polymorphonuclear leukocytes and monocytes. Following purification, leukocyte migration appeared in one bacteriocin fraction, and this migration was dependent on the concentration gradient in dilution ranges from 1/960 to 1/15. Chemotactic activity of the bacteriocin was heat labile and trypsin sensitive. Moreover, preincubation of bacteriocin with varying dilutions of its antiserum prepared in rabbits resulted in a significant loss of the chemotactic activity.

Keywords

Public Health Internal Medicine Biological Activity Trypsin Concentration Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Reeves, P. 1965. The bacteriocins.Bacteriol. Rev. 29:24–45.Google Scholar
  2. 2.
    Bradley, D. E. 1967. Ultrastructure of bacteriophages and bacteriocins.Bacteriol. Rev. 31:230–341.Google Scholar
  3. 3.
    Kelstrup, J., andR. J. Gibbons. 1969. Bacteriocins from human and rodent streptococci.Arch. Oral Biol. 14:251–258.Google Scholar
  4. 4.
    Paul, D., andH. D. Slade. 1975. Production and properties of an extracellular bacteriocin fromStreptococcus mutans bacteriocidal for group A and other streptococci.Infect. Immun. 12:1375–1385.Google Scholar
  5. 5.
    Fukushima, H., Y. Tsuboi, S. Fukushima, andH. Sagawa. 1980. The elimination of bacteriocin production in the oral bacteriumStreptococcus mutans.Arch. Oral Biol. 25:767–771.Google Scholar
  6. 6.
    Fukushima, H., S. Fukushima, T. Umemoto, H. Fukuhara, andH. Sagawa. 1982. Purification and chemical analysis of a bacteriocin from the oral bacteriumStreptococcus mutans Rm-10.Arch. Oral Biol. 27:721–727.Google Scholar
  7. 7.
    Fukushima, H., J. Kelstrup, S. Fukushima, T. Umemoto, A. Kaibori, andH. Sagawa. 1985. Characterization and mode of action of a purified bacteriocin from the oral bacteriumStreptococcus mutans RM-10.Arch. Oral Biol. 30:229–234.Google Scholar
  8. 8.
    Shiffmann, E., B. A. Corcoran, andS. M. Wahl. 1975. N-Formylmethionyl peptides as chemoattractants for leucocytes.Proc. Natl. Acad. Sci. USA 72:1059–1062.Google Scholar
  9. 9.
    Harvath, L., W. Falk, andE. J. Leonard. 1980. Rapid quantitation of neutrophil chemotaxis: Use of a polyvinylpyrrolidone-free polycarbonate membrane in a multiwell assembly.J. Immunol. Methods 37:39–45.Google Scholar
  10. 10.
    Falk, W., R. H. Goodwin, Jr., andE. J. Leonard. 1980. A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migration.J. Immunol. Methods 33:239–247.Google Scholar
  11. 11.
    Snyderman, R., L. C. Altman, M. S. Hausman, andS. E. Mergenhagen. 1972. Human mononuclear leukocyte chemotaxis: A quantitative assay for humoral and cellular chemotactic factors.J. Immunol. 108:857–860.Google Scholar
  12. 12.
    Böyum, A. 1968. Isolation of mononuclear cells and granulocytes from human blood. Isolation of mononuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at lg.Scand. J. Clin. Lab. Invest. 21:77–89.Google Scholar
  13. 13.
    Zigmond, S. H. 1977. Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors.J. Cell Biol. 75:606–616.Google Scholar
  14. 14.
    Wilkinson, P. C., J. F. Borel, V. J. Strecher-Levin, andE. Sorkin. 1969. Macrophage and neutrophil specific chemotactic factors in serum.Nature 222:244–247.Google Scholar
  15. 15.
    Snyderman, R., J. K. Phillips, andS. E. Mergenhagen. 1970. Polymorphonuclear leukocyte chemotactic activity in rabbit serum and guinea pig serum treated with immune complexes: Evidence for C5a as the major chemotactic factor.Infect. Immun. 1:521–525.Google Scholar
  16. 16.
    Gallin, J. I., R. A. Clark, andM. M. Frank. 1975. Kinetics analysis of chemotactic factor generation in human serum via activation of the classical and alternate complement pathways.Clin. Immunol. Immunopathol. 3:334–346.Google Scholar
  17. 17.
    Gallin, J. I., andA. P. Kaplan. 1974. Mononuclear cell chemotactic activity of kallikrein and plasminogen activatory and its inhibitory by Cl inhibitor and α2-macroglobulin.J. Immunol. 113:1928–1934.Google Scholar
  18. 18.
    Wahl, S. M., L. C. Altman, J. J. Oppenheim, andS. E. Mergenhagen. 1974.In vitro studies of a chemotactic lymphokine in the guinea pig.Int. Arch. Allergy 46:768–784.Google Scholar
  19. 19.
    Ward, P. A., C. D. Offen, andJ. R. Montgomery. 1971. Chemoattractants of leukocytes, with special reference to lymphocytes.Fed. Proc. 30:1721–1724.Google Scholar
  20. 20.
    Postlethwaite, A. E., andA. H. Kang. 1976. Collagen- and collagen peptide-induced chemotaxis of human blood monocytes.J. Exp. Med. 143:1299–1307.Google Scholar
  21. 21.
    Tainer, J. A., S. R. Turner, andW. S. Lynn. 1975. New aspects of chemotaxis. Specific target-cell attraction by lipid and lipoprotein fractions ofEscherichia coli chemotactic factor.Am. J. Pathol. 81:401–410.Google Scholar
  22. 22.
    Russel, R. J., R. J. McInroy, P. C. Wilkinson, andR. G. White. 1976. A lipid chemotactic factor from anaerobic coryneform bacteria includingCorynebacterium parvum with activity for macrophages and monocytes.Immunology 30:935–949.Google Scholar
  23. 23.
    Schiffmann, E., H. V. Showell, B. A. Corcoran, P. A. Ward, E. Smith, andE. L. Becker. 1975. The isolation and partial characterization of neutrophil chemotactic factors fromEscherichia coli.J. Immunol. 114:1831–1837.Google Scholar
  24. 24.
    Sveen, K. 1977. Rabbit polymorphonuclear leukocyte migrationin vitro in response to lipopolysaccharides fromBacteroides, Fusobacterium andVeillonella. Acta Pathol. Microbiol.Scand. Sect. B 85:374–380.Google Scholar
  25. 25.
    Nakajima, T., T. Yasuhara, S. Uzu, K. Wakamatsu, T. Miyazawa, K. Fukuda, andY. Tsukamoto. 1985. Wasp venom peptides; Wasp kinins, new cytotrophic peptide families and their physico-chemical properties.Peptides 6:425–430.Google Scholar
  26. 26.
    Nakajima, T., S. Uzu, K. Wakamatsu, K. Saito, T. Miyazawa, T. Yasuhara, Y. Tsukamoto, andM. Fujino. 1986. Amphiphilic peptides in wasp veom.Biopolymers 25:S115-S121.Google Scholar
  27. 27.
    Nelson, R. D., P. G. Quie, andR. L. Simmons. 1975. Chemotaxis under agarose: A new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes.J. Immunol. 115:1650–1656.Google Scholar

Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • Yoshio Tsukamoto
    • 1
  • Sachiko Fukutani
    • 1
  • Yasuyuki Takayama
    • 1
  • Hisanori Fukushima
    • 2
  • Hirosuke Sagawa
    • 2
  • Masakazu Mori
    • 1
  1. 1.Department of PharmacologyOsaka Dental UniversityOsakaJapan
  2. 2.Department of BacteriologyOsaka Dental UniversityOsakaJapan

Personalised recommendations