Light sensitivity of then-alkane-induced fatty alcohol oxidase fromCandida tropicalis andYarrowia lipolytica
Article
Received:
Accepted:
- 57 Downloads
- 10 Citations
Summary
Fatty alcohol oxidase (FAOD) activities in microsomal membrane fractions fromn-alkane-grownCandida tropicalis andYarrowia lipolytica rapidly decreased on exposure to light. The rate of inactivation of the enzyme depended upon the intensity and wavelength of the incident light, but was diminished under anaerobic conditions. Partially purified and solubilised FAOD preparations remained photosensitive: FAOD fromY. lipolytica was more photo-labile than that fromC. tropicalis.
Keywords
Enzyme Alcohol Anaerobic Condition Incident Light Membrane Fraction
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Preview
Unable to display preview. Download preview PDF.
References
- Blasig R, Mauersberger S, Riege P, Schunk W-H, Jockisch W, Franke P (1988) Degradation of long chainn-alkanes by the yeastCandida maltosa. Appl Microbiol Biotechnol 28:589–597Google Scholar
- Il'Chenko AP (1984) Oxidase of higher alcohols in the yeastTorulopsis candida grown on hexadecane. Mikrobiologiya 53:903–906Google Scholar
- Kemp GD, Dickinson FM, Ratledge C (1988) Inducible long chain alcohol oxidase from alkane-grownCandida tropicalis. Appl Microbiol Biotechnol 29:370–374Google Scholar
- Krauss W, Schiebel G, Eberl D, Hager A (1987) Blue light induced, reversible inactivation of the tonoplast-type H+-ATPase from corn coleoptiles in the presence of flavins. Photochem Photobiol 45:837–844Google Scholar
- Krauzova VI, Il'Chenko AP, Sharyshev AA, Lozinov AB (1985) Possible pathways of the oxidation of higher alcohols by membrane fractions of yeasts cultured on hexadecane and hexadecanol. Biokhimiya 50:726–732Google Scholar
- Krauzova VI, Kuvichkina TN, Sharyshev AA, Romanova IB, Lozinov AB (1986) Formation of lauric acid and NADH in the oxidation of dodecanol by membrane fractions of the yeastCandida maltosa, cultured on hexadecane. Biokhimiya 51:23–27Google Scholar
- Lebeault JM, Roche B, Duvjnac Z, Azoulay E (1970) Isolation and study of the enzymes involved in the metabolism of hydrocarbons byCandida tropicalis. Arch Microbiol 72:140–153Google Scholar
- Senger H (1982) The effect of blue light on plants and microorganisms. Photochem Photobiol 35:911–920Google Scholar
- Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150:76–85Google Scholar
- Yamada T, Nawa H, Kawamoto S, Tanaka A, Fukui S (1980) Subcellular localisation of long-chain alcohol deyhdrogenase and aldehyde dehydrogenase inn-alkane grownCandida tropicalis. Arch Microbiol 128:145–151Google Scholar
Copyright information
© Springer-Verlag 1990