Applied Microbiology and Biotechnology

, Volume 42, Issue 2–3, pp 385–390 | Cite as

Antifungal activity of cysteine, its effect on C-21 oxygenated lanosterol derivatives and other lipids in Inonofus obliquus, in vitro

  • K. Kahlos
  • V. H. Tikka
Original Paper


The antifungal activity of cysteine (2-amino-3-mercaptopropionic acid) on mycelial growth and the production of lanosterol, 3,β-hydroxy-lanosta-8,24-dien-21-al, 3,β-21,dihydroxy-lanosta-8,24-diene, trametenolic acid and ergosterol were investigated in solid and liquid cultures ofInonotus obliquus at cysteine concentrations of 0.9 and 9.0 mm by means of thinlayer chromatography densitometry. Cysteine elicited the production of ergosterol in all the cultures and at a concentration of 0.9 mm distinctly elicited the production of 3,β-hydroxy-lanosta-8,24-dien-21-al. At 9.0 mm, cysteine had greater antifungal activity than at 0.9 mm, decreasing growth and the production of metabolities.


Oxygenate Lipid Chromatography Cysteine Thinlayer Chromatography 
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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • K. Kahlos
    • 1
  • V. H. Tikka
    • 1
  1. 1.Department of PharmacyUniversity of Helsinki, Pharmacognosy DivisionFabianinkatu 35Finland

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