Applied Microbiology and Biotechnology

, Volume 42, Issue 2–3, pp 375–384 | Cite as

Stability of pressure-extruded liposomes made from archaeobacterial ether lipids

  • C. G. Choquet
  • G. B. Patel
  • G. D. Sprott
  • T. J. Beveridge
Original Paper


Ether lipids were obtained from a wide range of archaeobacteria grown at extremes of pH, temperature, and salt concentration. With the exception ofSulfolobus acidocaldarius, unilamellar and/or multilamellar liposomes could be prepared from emulsions of total polar lipid extracts by pressure extrusion through filters of various pore sizes. Dynamic light scattering, and electron microscopy revealed homogeneous liposome populations with sizes varying from 40 to 230 nm, depending on both the lipid source and the pore size of the filters. Leakage rates of entrapped fluorescent or radioactive compounds established that those archaeobacterial liposomes that contained tetraether lipids were the most stable to high temperatures, alkaline pH, and serum proteins. Most ether liposomes were stable to phospholipase A2, phospholipase B and pancreatic lipase. These properties of archaeobacterial liposomes make them attractive for applications in biotechnology.


Lipid Lipase Pore Size Salt Concentration Dynamic Light Scattering 
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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • C. G. Choquet
    • 1
  • G. B. Patel
    • 1
  • G. D. Sprott
    • 1
  • T. J. Beveridge
    • 2
  1. 1.National Research Council of Canada (NRCC)Institute for Biological SciencesOttawaOntarioCanada
  2. 2.Department of Microbiology, College of Biological ScienceUniversity of GuelphGuelph, OntarioCanada

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