Storing of triethylene melamine-treated mature spermatozoa in untreated females was found to result in increased frequencies of both sex-linked recessive lethals and translocations involving the Y, II and III chromosomes. Frequencies of these mutations in effectively unstored spermatozoa were determined from progenies produced using sperm 2–4 days after treatment. The increase in translocation frequencies was on the order of 12-fold in progenies from sperm utilized 11–13 days after treatment when the sperm were stored at 25°C, and 3- to 6-fold when comparable sperm were stored at 12.5°C. Consistent but much smaller increases in frequencies of sex-linked lethals were found, with the increase in lethals tending to be correlated with relative increase in translocation frequency in a given experiment. On the assumption that sex-linked lethals related to chromosome breakage would be expected to increase in frequency in the same proportion as do translocations, approximate agreement was obtained when the proportions of breakage-related lethals among unstored lethals were estimated from the data in the four experimental series. The data are thus consistent with the hypothesis that chromosome breaks but not point mutations are realized during storage of treated spermatozoa. Possible interpretations of a differential effect of storage on treated chromosomes are discussed.
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Auerbach, C., andE. M. Sonbati: Sensitivity of theDrosophila testis to the mutagenic action of mustard gas. Z. Vererb.-Lehre91, 237 (1960).
Brookes, P., J. A. V. Butler, D. J. R. Laurence, P. D. Lawley andK. V. Shooter: Investigations of degradative reactions following alkylation of DNA by mustard gas and half-S-mustard. Ann. Rep. British Empire Cancer Campaign38, 6 (1960).
Fahmy, O. G., andM. J. Bird: Chromosome breaks among recessive lethals induced by chemical mutagens inDrosophila melanogaster. Heredity6, (Suppl.), 149 (1953).
Fano, U.: Note on the theory of radiation-induced lethals inDrosophila. Science106, 87 (1947).
Herskowitz, I. H.: The relationship of X-ray induced recessive lethals to chromosomal breakage. Amer. Naturalist80, 588 (1946).
—: The incidence of chromosomal rearrangements and recessive lethal mutations following treatment of matureDrosophila sperm with 2:4:6-tri(ethylene-imino)-1:3:5-triazine. Genetics40, 574 (1955).
—: Mutagenesis in matureDrosophila spermatozoa by “triazine” applied in vaginal douches. Genetics41, 605 (1956).
Lawley, P. D.: The action of alkylating agents on deoxyribonucleic acid. J. chim. Phys.58, 1011 (1961).
Lea, D. E., andD. G. Catcheside: The relation between recessive lethals, dominant lethals, and chromosome rearrangements inDrosophila. J. Genet.47, 10 (1945).
Lorkiewicz, Z., andW. Szybalski: Mechanism of chemical mutagenesis IV. Reaction between triethylene melamine and nucleic acid components. J. Bact.82, 195 (1961).
Muller, H. J.: Résumé and perspectives of the symposium on genes and chromosomes. Cold Spr. Harb. Symp. Quant. Biol.9, 290 (1941).
—: The manner of production of mutations by radiation. In: Radiation biology,A. Hollaender, ed., vol.1, chap.7. New York: McGraw-Hill Book Co. 1954.
—: On the relation between chromosome changes and gene mutations. Brookhaven Symp. Biol.8, 126 (1956).
—, andE. Altenburg: The frequency of translocations produced by X-rays inDrosophila. Genetics15, 283 (1930).
Schalet, A.: The relationship between the frequency of nitrogen mustard induced translocations in mature sperm ofDrosophila and utilization of sperm by females. Genetics40, 594 (1955).
—: A second case of translocation between paternal and maternal chromosomes. Amer. Naturalist90, 329 (1956).
Szybalski, W.: The mechanism of chemical mutagenesis with special reference to triethylene melamine action. In: Developments in industrial microbiology,B. M. Miller, ed., vol. 1, p. 231. New York: Plenum Press 1960.
Taylor, J. H.: The replication and organization of DNA in chromosomes. In: Molecular genetics,J. H. Taylor, ed., part 1, chap. 2 New York: McGraw-Hill Book Co. 1963.
Yanders, A. F.: Relative time of eclosion ofDrosophila females heterozygous for sex-linked recessive lethals. Amer. Naturalist92, 189 (1958).
Studies carried out while the author was a guest investigator at the Institute of Animal Genetics on sabbatical leave from the University of Minnesota.
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Snyder, L.A. Evidence of an essential difference between point mutations and chromosome breaks induced by triethylene melamine inDrosophila spermatozoa. Zeitschrift für Vererbungslehre 94, 182–189 (1963). https://doi.org/10.1007/BF00895895
- Point Mutation
- Differential Effect
- Relative Increase
- Experimental Series