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The distribution and taxonomic value of fatty acids and eicosanoids in the Lipomycetaceae and Dipodascaceae

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Using radioimmunoassay, blood platelet aggregation studies and GC-MS the existence of prostaglandins in the endomycetalean yeastDipodascopsis uninucleata was confirmed by our group. These findings triggered the search for similar eicosanoids in the rest of the Endomycetales. We commenced by scanning for the easily detectable precursors of eicosanoids, linoleic- and linolenic acid. We selected two families (i.e. Lipomycetaceae and Dipodascaceae), both producing these precursors, for further investigation.

Representative strains of the two families were tested for their ability to grow in the presence of 1mM aspirin, a specific inhibitor of prostaglandin biosynthesis. In contrast to the lipomycetaceous species the dipodascaceous species were insensitive to this drug. These results were verified when representative strains of both families were investigated for their ability to produce eicosanoids from externally fed radio-labeled arachidonic acid along an aspirin sensitive pathway. Thin layer chromatography of culture extracts, followed by autoradiography, showed that while none of the Dipodascaceae produced aspirin sensitive arachidonic acid metabolites, the members of the Lipomycetaceae tested positive for these metabolites. These findings supported the separation of the lipomycetaceous yeastDipodascopsis from the Dipodascaceae. The findings also correlate with the delimitation of these yeasts in two families (i.e. Dipodascaceae and Lipomycetaceae).

Further investigation indicated that prostaglandin production by the genusDipodascopsis is mainly associated with ascosporogenesis. Thin layer chromatography of cell extracts fromDipodascopsis tóthii, followed by scintillation counting, indicated the presence of PGF and PGE2 during ascosporogenesis.

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  1. Augustyn OPH, Kock JLF & Ferreira D (1990) Differentiation between yeast species, and strains within a species, by cellular fatty acid analyses. 3.Saccharomyces sensu lato, Arxiozyma andPachytichospora. System. Appl. Microbiol. 13: 44–55

  2. Barnett JA, Payne RW & Yarrow D (1990) Yeasts characteristics and identification. 2nd ed., Cambridge University Press, Cambridge

  3. Benedetto C, McDonald-Gibson RG, Nigam S & Slater TF (1987) Prostaglandins and related substances. IRL Press, Oxford

  4. Biggs R (1937)Dipodascus uninucleatus. Mycologia 29: 34–44

  5. Botha A, Kock JLF, Van Dyk MS, Coetzee DJ, Augustyn OPH & Botes PJ (1993) Yeast Eicosanoids IV. Evidence for Prostaglandin Production during Ascosporogenesis byDipodascopsis tóthii (submitted)

  6. Botha A, Kock JLF, Coetzee DJ, Van Dyk MS, Van der Berg L & Botes PJ (1992a) Yeast eicosanoids I. The distribution and taxonomic value of cellular fatty acids and arachidonic acid metabolites in the Dipodascaceae and related taxa. System. Appl. Microbiol. 15: 148–154

  7. Botha A, Kock JLF, Coetzee DJ, Van der Linde NA & Van Dyk MS (1992b) Yeast eicosanoids II. The influence of non-steroidal anti-inflammatory drugs on the life cycle ofDipodascopsis. System. Appl. Microbiol. 15: 155–160

  8. Cottrell M & Kock JLF (1989) The yeast family Lipomycetaceae Novak et Zsolt emend. Van der Walt et al. and the genusMyxozyma Van der Walt et al. 1. A historical account of its delimitation and 2. The taxonomic relevance of cellular long-chain fatty acid composition and other phenotypic characters. System. App. Microbiol. 12: 291–305

  9. Cottrell M, Kock JLF, Lategan PM, Botes PJ & Britz TJ (1985) The long-chain fatty acid compositions of species representing the genusKluyveromyces. FEMS Microbiol. Lett. 30: 373–376

  10. Cottrell M, Viljoen BC, Kock JLF & Lategan PM (1986) The long-chain fatty acid compositions of species representing the generaSaccharomyces, Schwanniomyces andLipomyces. Short Comm. J. Gen. Microbiol. 132: 2401–2403

  11. De Hoog GS, Smith MTh & Gueho E (1986) A revision of the genusGeotrichum and its teleomorphs. Stud. Mycol. 29: 1–131

  12. Doi S, Watanabe M & Yoshimuta M (1988) Arachidonic and oleic acids stimulate zygote formation in the presence of mating pheromone in the yeastSaccharomyces cerevisiae. Arch. Microbiol. 149: 507–508

  13. Golubev WI, Smith MT, Poot GA & Kock JLF (1989) Species delineation in the genusNadsonia Sydow. Antonie van Leeuwenhoek 55: 369–382

  14. Gregson RP, Marwood JF & Quinn RJ (1979) The occurance of prostaglandins PGE2 and PGF in plant — the red algaGracilaria lichenoides. Tetrahedron Lett. 46: 4505–4506

  15. Herman RP & Luchini MM (1989) Lipoxygenase activity in the oomyceteSaprolegnia is dependant on environmental cues and reproductive competence. Experimental Mycology 13: 372–379

  16. Herman RP & Herman CA (1985) Prostaglandins or prostaglandin like substances are implicated in normal growth and development in oomycetes. Prostaglandins 29: 819–830

  17. Kock JLF (1988) Chemotaxonomy and yeasts. S. Afr. J. Sci. 84: 735–740

  18. Kock JLF, Botes PJ, Erasmus SC & Lategan PM (1985) A rapid method to differentiate between four species of the Endomycetaceae. Short Comm. J. Gen. Microbiol. 131: 3393–3396

  19. Kock JLF, Erasmus SC & Lategan PM (1986) A rapid method to identify some species of the Dipodascaceae and Endomycetaceae. J. Microbiol. Meth. 5: 199–203

  20. Kock JLF, Coetzee DJ, Van Dyk MS. Truscott M, Botha A & Augustyn OPH (1992) Evidence for, and taxonomic value of, an arachidonic acid cascade in the Lipomycetaceae. Antonie van Leeuwenhoek (in press)

  21. Kock JLF, Coetzee DJ, Van Dyk MS, Truscott M, Cloete FG, Van Wyk V & Augustyn OPH (1991) Evidence for pharmacologically active prostaglandins in yeasts. S. Afr. J. Sci. 87: 73–76

  22. Kreger-van Rij NJW (1974) The speciesDipodascopsis uninucleata (Biggs) Batra & Millner. Persoonia 8: 105–109

  23. Kreger-van Rij NJW & Veenhuis M (1974) Spores and septa in the genusDipodascus. Can. J. Bot. 52: 1335–1338

  24. Miller M, Kock JLF & Botes PJ (1989) The significance of long-chain fatty acid compositions and other phenotypic characteristics in the genusPichia Hansenemend. Kurtzman. System. Appl. Microbiol. 12: 70–79

  25. Muller HB & Kock JLF (1986)Waltiozyma gen. nov. (Saccharomycetaceae), a new genus of the Endomycetales. S. Afr. J. Sci. 82: 491–492

  26. Nomura T & Ogata H (1976) Distribution of prostaglandins in the animal kingdom. Biochimica et Biophysica Acta 431: 127–131

  27. Ogburn PL & Brenner WE (1981) The physiologic actions and effects of prostaglandins. Upjohn Co., Kalamazoo

  28. Oosthuizen A, Kock JLF, Botes PJ & Lategan PM (1987) The long-chain fatty acid composition of yeasts used in the brewing industry. Appl. Microbiol. Biotechnol. 26: 55–60

  29. Panossian AG (1987) Search of prostaglandins and related compounds in plants. Prostaglandins 33: 363–381

  30. Robinson HJ & Vane JR (1974) Prostaglandin synthetase inhibitors — their effects on physiological functions and pathological states. Raven Press, New York

  31. Schechter G & Grossman S (1983) Lipoxygenase from baker's yeast: Purification and properties. Int. J. Biochem. 15: 1295–1304

  32. Van der Walt JP, Wingfield MJ, Smith MTh & Batenburg-van der Vegte WH (1991) Septal microspores inZygozyma and their taxonomic significance. Antonie van Leeuwenhoek 59: 77–80

  33. Van der Walt JP & Yarrow D (1984) Methods for the isolation, maintenance, classification and identification of yeasts. In: Kreger-van Rij NJW (Ed) The Yeasts, a Taxonomic Study, 3rd ed. (pp 45–104) Elsevier Sci. Publ., Amsterdam

  34. Van Dyk MS, Kock JLF, Coetzee DJ, Augustyn OPH & Nigam S (1991) Isolation of a noval arachidonic acid metabolite 3-hydroxy 5,8,11,14-eicosatetraenoic acid (3-HETE) from the yeastDipodascopsis uninucleata UOFS-Y128. FEBS Lett. 283: 195–198

  35. Viljoen BC, Kock JLF & Lategan PM (1986) Long-chain fatty acid composition of selected genera of yeasts belonging to Endomycetales. Antonie van Leeuwenhoek 52: 45–51

  36. Viljoen BC, Kock JLF & Thoupou K (1989) The significance of cellular long-chain fatty acid compositions and other criteria in the study of the relationship between sporogenous ascomycete species and asporogenousCandida species. System. Appl. Microbiol. 12: 80–90

  37. Von Arx JA & van der Walt JP (1987) Ophiostomatales and Endomycetales. In: De Hoog GS, Smith MTh & Weijman ACM (Eds) The Expanding Realm of Yeast-like Fungi (pp 165–176) Elsevier Sci. Publ., Amsterdam

  38. Weijman ACM & van der Walt JP (1989) Carbohydrate profiles and the taxonomic concept of the Lipomycetaceae. Stud. Mycol. 31: 193–199

  39. Zsolt J (1963) Morphological and physiological investigations of aDipodascus strain isolated in Hungary. Acta Botanica Academiae Scientiarum Hungaricae 9: 217–226

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Botha, A., Kock, J.L.F. The distribution and taxonomic value of fatty acids and eicosanoids in the Lipomycetaceae and Dipodascaceae. Antonie van Leeuwenhoek 63, 111–123 (1993). https://doi.org/10.1007/BF00872387

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Key words

  • taxonomy
  • fatty acids
  • eicosanoids
  • Lipomycetaceae
  • Dipodascaceae