Antonie van Leeuwenhoek

, Volume 65, Issue 3, pp 245–250 | Cite as

Strategies for improving heterologous protein production from filamentous fungi

  • David B. Archer
  • David J. Jeenes
  • Donald A. Mackenzie


Despite the naturally high capacity for protein secretion by many species of filamentous fungi, secteted yields of many heterologous proteins have been comparatively low. The strategies for yield improvement have included the use of strong homologous promoters, increased gene copy number, gene fusions with a gene encoding a naturally well-secreted protein, protease-deficient host strains and screening for high yields following random mutagenesis. Such approaches have been effective with some target heterologous proteins but not others.

Approaches used in heterologous protein production from filamentous fungi are discussed and a perspective on emerging strategies is presented.

Key words

Aspergillus gene expression heterologous protein protein secretion Trichoderma 


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • David B. Archer
    • 1
  • David J. Jeenes
    • 1
  • Donald A. Mackenzie
    • 1
  1. 1.Institute of Food ResearchColney, NorwichUK

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