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Hexavalent-chromium reduction by a chromate-resistantBacillus sp. strain

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Abstract

Bacillus strain QC1-2, isolated from a chromium-polluted zone, was selected by its high ability to both tolerate and reduce hexavalent chromium [Cr(VI)] to less-toxic trivalent chromium [Cr(III)]. Cell suspensions of strain QC1-2 rapidly reduced Cr(VI), in both aerobic and anaerobic conditions, to Cr(III) which remained in the supernatant. Cr(VI) reduction was dependent on the addition of glucose but sulfate, an inhibitor of chromate transport, had no effect. Studies with permeabilized cells and cell extracts showed that the Cr(VI) reductase of strain QC1-2 is a soluble NADH-dependent enzyme.

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References

  1. American Public Health Association (1971) Chromium, pp 115–159. In: Standard Methods for the Examination of Water and Wastewater. Amer. Public Health Assoc., New York

  2. Basabe JR, Lee CA & Weiss RL (1979) Enzyme assays using permeabilized cells ofNeurospora. Anal. Biochem. 92: 356–360

  3. Bopp LH & Ehrlich HL (1988) Chromate resistance and reduction inPseudomonas fluorescens LB300. Arch. Microbiol. 150: 426–431

  4. Cervantes C (1991) Bacterial interactions with chromate. Antonie van Leeuwenhoek 59: 229–233

  5. Cervantes C & Ohtake H (1988) Plasmid-determined chromate resistance inPseudomonas aeruginosa. FEMS Microbiol. Lett. 56: 173–176

  6. Cervantes C & Silver S (1992) Bacterial chromate resistance and chromate reduction. Plasmid 27: 65–71

  7. Das S & Chandra AL (1990) Chromate reduction inStreptomyces. Experientia 46: 731–733

  8. Gvozdyak PL, Mogilavich NF, Rylskii AF & Grishchenko NI (1986) Reduction of hexavalent chromium by collection strains of bacteria. Mikrobiologiya 55: 962–965

  9. Holt JG (Ed.) (1977) The shorter Bergey's Manual of Determinative Microbiology. 8th Ed. Williams & Wilkins, Baltimore

  10. Horitsu H, Futo S, Miyazawa Y, Ogai S & Kawai K (1987) Enzymatic reduction of hexavalent chromium by hexavalent chromium tolerantPseudomonas ambigua G-1. Agric. Biol. Chem. 51: 2417–2420

  11. Ishibashi Y, Cervantes C & Silver S (1990) Chromium reduction inPseudomonas putida. Appl. Environ. Microbiol. 56: 2268–2270

  12. Lowry OH, Rosebrough NJ, Farr AL & Randall RJ (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275

  13. Ohtake H, Cervantes C & Silver S (1987) Decreased chromate uptake inPseudomonas fluorescens carrying a chromate resistance plasmid. J. Bacteriol. 169: 3853–3856

  14. Suzuki T, Miyata N, Horitsu H, Kawai K, Takamizawa K, Tai Y & Okazaki M (1992) NAD(P)H-dependent chromium (VI) reductase ofPseudomonas ambigua G-1: a Cr(V) intermediate is formed during the reduction of Cr(VI) to Cr(III). J. Bacteriol. 174: 5340–5345

  15. Wang PC, Mori T, Komori K, Sasatsu M, Toda K & Ohtake H (1989) Isolation and characterization of anEnterobacter cloacae strain that reduces hexavalent chromium under anaerobic conditions. Appl. Environ. Microbiol. 55: 1665–1669

  16. Wang PC, Mori T, Toda K & Ohtake H (1990) Membrane-associated chromate reductase activity fromEnterobacter cloacae. J. Bacteriol. 172: 1670–1672

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Correspondence to Carlos Cervantes.

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Campos, J., Martinez-Pacheco, M. & Cervantes, C. Hexavalent-chromium reduction by a chromate-resistantBacillus sp. strain. Antonie van Leeuwenhoek 68, 203–208 (1995). https://doi.org/10.1007/BF00871816

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Key words

  • chromate reduction
  • chromate resistance
  • Bacillus