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Characterization of the heat shock response inEnterococcus faecalis

Abstract

We have characterized the general properties of the heat shock response of the Gram-positive hardy bacteriumEnterococcus faecalis. The heat resistance (60°C or 62.5°C, 30 min) of log phase cells ofE. faecalis grown at 37°C was enhanced by exposing cells to a prior heat shock at 45°C or 50°C for 30 min. These conditioning temperatures also induced ethanol (22%, v/v) tolerance. The onset of thermotolerance was accompanied by the synthesis of a number of heat shock proteins. The most prominent bands had molecular weights in the range of 48 to 94kDa. By Western blot analysis two of them were found to be immunologically related to the well known DnaK (72 kDa) and GroEL (63 kDa) heat shock proteins ofEscherichia coli. Four other proteins showing little or no variations after exposure to heat are related to DnaJ, GrpE and Lon (La)E. coli proteins and to theBacillus subtilis δ43 factor. Ethanol (2% or 4%, v/v) treatments elicited a similar response although there was a weaker induction of heat shock proteins than with heat shock.

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References

  1. Anderson CD & McKay LL (1983) Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl. Environm. Microbiol. 46: 549–552

  2. Barnes CA, Johnston GC & Singer RA (1990) Thermotolerance is independent of induction of the full spectrum of heat shock proteins and a cell cycle blockage in the yeast. J. Bacteriol. 172: 4352–4358

  3. Board RG (1983) A Modern Introduction to Food Microbiology. Blackwell Scientific Publications, Oxford

  4. Bosch TCG, Krylow SM, Bodeh R & Steeler E (1988) Thermotolerance and synthesis of heat shock proteins: these responses are present inHydra attenwata but absent inHydra oligactis. Proc. Natl. Acad. Sci. USA 85: 7927–7931

  5. Boutibonnes P, Gillot B, Auffray Y & Thammavongs B (1991) Heat-shock induces thermotolerance and inhibition of lysis in a lysogenic strain ofLactococcus lactis. Int. J. Food. Microbiol. 14: 1–10.

  6. Boutibonnes P, Tranchard C, Hartke A, Thammavongs B & Auffray Y (1992) Is thermotolerance correlated to heat shock protein synthesis inLactococcus lactis. Int. J. Food. Microbiol. 16: 227–236

  7. Bunning VK, Crawford RG, Tierney JT & Peeler JT (1992) Thermotolerance of heat-shockedListeria monocytogenes in milk exposed to high temperature short time pasteurization. Appl. Environ. Microbiol. 58: 2096–2098.

  8. Ellis RJ & Van der Vies SM (1991) Molecular Chaperones. Ann. Rev. Biochem. 60: 321–347

  9. Engel JN, Pollack J, Perara E & Ganem D (1990) Heat shock response of murineChlamydia trachomatis. J. Bacteriol. 172: 6959–6972

  10. Georgopoulos C, Ang P, Liberek K & Zylicz M (1990) Properties of theEscherichia coli heat shock proteins and their role in bacteriophage λ growth. In: Morimoto RI, Tissières A & Georgopoulos C (Eds) Stress Proteins in Biology and Medicine (pp 191–221) Cold Spring Harbor Lab. Press, New York

  11. Hahn GM & Li GC (1990) Thermotolerance, thermoresistance and thermosensitization. In: Morimoto RI, Tissières A & Georgopoulos C (Eds) Stress Proteins in Biology and Medicine (pp 79–100) Cold Spring Harbor Lab. Press, New York

  12. Laszlo A (1988) Evidence for two states of thermotolerance in mammalian cells. Int. J. Hypertermia 4: 513–526

  13. Lindquist S (1986) The heat shock response. Ann. Rev. Biochem. 55: 1151–1191

  14. Lindquist S & Craig EA (1988) The heat-shock proteins. Ann. Rev. Genet. 26: 631–677

  15. Mackey BM & Derrick C (1990) Heat shock protein synthesis and thermotolerance inSalmonella typhimurium. J. Appl. Bacteriol. 69: 373–383

  16. Morimoto RI, Tissières A & Georgopoulos C (1990) Stress Proteins in Biology and Medicine. Cold Spring Harbor Laboratory Press, New York

  17. Nagao RT, Kimpem JA & Key LJL (1990) Molecular and cellular biology of the heat shock response. In: Scandalios JG (Ed) Genomic Response to Environmental Stress (pp 235–274) Academic Press, Boston

  18. Neidhardt FC & VanBogelen RA (1987) Heat shock response. In: Neidhardt FC (Ed)Escherichia coli andSalmonella typhimurium, Cellular and Molecular Biology, Vol 2 (pp 1334–1345) Library of Congress, Washington

  19. Sanchez Y & Lindquist SL (1990) Hsp104 is required for induced thermotolerance. Science 248: 112–115

  20. Sanchez Y, Taulien J, Borkovich KA & Lindquist S (1992) Hsp104 is required for tolerance to many forms of stress. The Embo J. 11: 2357–2364

  21. Skinner FA & Quesnel LB (1978) Streptococci. Academic Press, London, 415pp

  22. Stamm LV, Gherardini FC, Parrish EA & Moomaw CR (1991) Heat shock proteins in Spirochetes. Infect. Immun. 59: 1572–1575

  23. Terzaghi BE & Sandine WE (1975) Improved medium for lactic streptococci and their bacteriophages. Appl. Microbiol. 29: 807–813

  24. VanBogelen RA, Acton MA & Neidhardt FC (1987) Induction of the heat shock regulon does not produce thermotolerance inEscherichia coli. Genes Dev. 1: 525–531

  25. —— (1990) Ribosomes as sensors of heat and cold shock inEscherichia coli. Proc. Natl. Acad. Sci. USA 87: 5589–5593

  26. Weber LA (1992) Relationship of heat shock proteins and induced thermal resistance. Cell. Prolif. 25: 101–113

  27. Yamamori T & Yura T (1982) Genetic control of heat shock protein synthesis and its bearing on growth and thermal resistance inEscherichia coli K12. Proc. Natl. Acad. Sci. USA 79: 860–864

  28. Zschech KK & Murray BE (1990) Evidence for a staphylococcal like mercury resistance gene inEnterococcus faecalis. Antimicrob. Agents Chemother. 34: 1287–1289

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Boutibonnes, P., Giard, J.C., Hartke, A. et al. Characterization of the heat shock response inEnterococcus faecalis . Antonie van Leeuwenhoek 64, 47–55 (1993). https://doi.org/10.1007/BF00870921

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Key words

  • heat shock response
  • Enterococcus faecalis
  • heat shock proteins
  • thermotolerance