, Volume 29, Issue 12, pp 882–887 | Cite as

Stimulation of 2-deoxyglucose uptake in rat adipocytes by a human growth hormone fragment (hGH 4-15)

  • F. M. Ng
  • J. A. Harcourt


The in vivo and in vitro effects of a hypoglycaemic fragment of human growth hormone containing the sequence H2N-Ile-Pro- Leu-Ser-Arg-Leu-Phe-Asp-Asn-Ala-Met-Leu-COOH (hGH 4–15) on 2-deoxy-[1-14C]-D-glucose uptake in adipocytes were studied. The isolated cells from rats after a single intravenous injection of hGH 4–15 (1 mg/kg) significantly increased uptake of 2-deoxyglucose (p < 0.005). Adipocytes from untreated rats pre-incubated with the hGH fragment (10 μg/ml) at 37°C for 30 min also clearly showed an elevated uptake of 2-deoxyglucose in the absence and the presence of exogenous insulin. The effect of hGH 4–15 was concentration-dependent, steadily increasing with the maximum effect at 10 μg/ml. The present findings suggest that the enhancement of glucose transport in target tissues may be a major contributing factor to the hypoglycaemic action of the amino-terminal fragments of human growth hormone.

Key words

Human growth hormone synthetic fragment 4–15 glucose transport rat adipocytes 


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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • F. M. Ng
    • 1
  • J. A. Harcourt
    • 1
  1. 1.Department of Biochemistry, Faculty of MedicineMonash UniversityClaytonAustralia

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