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Loss of alleles in brain tumours: Distribution and correlations with clinical course

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Abstract

Gliomas (n = 44) and meningiomas (n = 24) of different grades of malignancy were analysed for allele losses at loci on chromosomes 10, 13, 17 and 22. Deletions of genetic material on these chromosomes occurred in gliomas without being restricted to any histological entity. The frequency of chromosome-l0-specific allele losses increased significantly with the age of the patients and with the grade of malignancy of the tumours. Deletions of chromosome 10 material were associated with a poor prognosis. The glioblastomas of patients aged over 70 years lacked the loss of the entire chromosome 10, even in tumours with EGFR gene amplification. Deletions at loci of chromosomes 13, 17 and 22 were observed in 18–32% of all gliomas, independent of grade of malignancy, patients' age, EGFR gene amplification and clinical course. Only chromosome-22-specific allele losses were found preferentially in gliomas of female patients. Loss of chromosome 22 alleles in 44% was the only mutation detected in meningiomas. This occurred independently of grade of malignancy and biological factors.

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References

  1. 1.

    Bragg T, Nakamura Y, Jones C, White R (1988) Isolation and mapping of a polymorphic DNA sequence (cTBQ7) on chromosome 10 [D10S28]. Nucleic Acids Res 16: 11395

  2. 2.

    Bragg T, Nakamura Y, Jones C, White R (1988) Isolation and mapping of a polymorphic DNA sequence (cTB 14.16) on chromosome 10 [D10S33]. Nucleic Acids Res 16: 11391

  3. 3.

    Clarke MF, Westin E, Schmidt D, Josephs SF, Ratner L, Wong-Staal F, Gallo RC, Reitz MS Jr (1984) Transformation of NIH 3T3 cells by a human c-sis cDNA clone. Nature 308: 464–466

  4. 4.

    Deimling A von, Louis DN, Ammon K von, Petersen I, Hoell T, Chung RY, Martuza RL, Schoenfeld DA, Yasargil MG, Wiestler OD, Seizinger BR (1992) Association of epidermal growth factor receptor gene amplification with loss of chromosome 10 in human glioblastoma multiforme. J Neurosurg 77: 295–301

  5. 5.

    Deimling A von, Ammon K von, Schoenfeld D, Wiestler OD, Seizinger BR, Louis DN (1993) Subsets of glioblastoma multiforme defined by molecular genetic analysis. Brain Pathol 3: 19–26

  6. 6.

    Diedrich U, Soja S, Behnke J, Zoll B (1991) Amplification of the c-erbB oncogene is associated with malignancy in primary tumours of neuroepithelial tissue. J Neurol 238: 221–224

  7. 7.

    Karlbom AE, James CD, Boethius J, Cavenee WK, Collins VP, Nordenskjöld M, Larsson C (1993) Loss of heterozygosity in malignant gliomas involves at least three distinct regions on chromosome 10. Hum Genet 92: 169–174

  8. 8.

    Keime S, Adham I, Engel W (1990) Nucleotide sequence and exon-intron organization of the human proacrosin gene. Eur J Biochem 190: 195–200

  9. 9.

    Kondoleon S, Vissing H, Luo XY, Magenis RE, Kellogg J, Litt M (1987) A hypervariable RFLP on chromosome 17p13 is defined by an arbitrary single copy probe p144-D6 [HGM9 No. D17S34]. Nucleic Acids Res 15: 10605

  10. 10.

    Krumlauf R, Jeanpierre M, Young BD (1982) Construction and characterization of genomic libraries from specific human chromosomes. Proc Natl Acad Sci USA 79: 2971–2975

  11. 11.

    Leenstra S, Troost D, Westerveld A, Bosch DA, Hulsebos TIM (1992) Molecular characterization of areas with low grade tumor or satellitosis in human malignant astrocytomas. Cancer Res 52: 1568–1572

  12. 12.

    Nakamura Y, Ballard L, Leppert M, O'Connell P, Lathrop GM, Lalouel JM, White R (1988) Isolation and mapping of a polymorphic DNA sequence (pYNZ22) on chromosome 17p [DS7S30]. Nucleic Acids Res 16: 5707

  13. 13.

    Rempel SA, Schwechheimer K, Davis RL, Cavenee WK, Rosenblum ML (1993) Loss of heterozygosity on chromosome 10 is associated with morphologically malignant meningioma progression. Cancer Res 53: 2386–2392

  14. 14.

    Watanabe K, Nagai M, Wakai S, Arai T, Kawashima K (1990) Loss of constitutional heterozygosity in chromosome 10 in human glioblastoma. Acta Neuropathol 80: 251–254

  15. 15.

    Wiggs J, Nordenskjöld M, Yandell D, Rapaport J, Grondin V, Janson M, Werelius B, Petersen R, Craft A, Riedel K, Liberfarb R, Walton D, Wilson W, Dryja TP (1988) Prediction of the risk of hereditary retinoblastoma, using DNA polymorphisms within the retinoblastoma gene. N Engl J Med 318:151–157

  16. 16.

    Zülch KJ (1979) Histological typing of tumours of the central nervous system. International classification of tumours no. 21. World Health Organisation, Geneva

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Correspondence to Uwe Diedrich.

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Supported by a grant of the Deutsche Krebshilfe

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Diedrich, U., Lucius, J., Bittermann, H. et al. Loss of alleles in brain tumours: Distribution and correlations with clinical course. J Neurol 242, 707–711 (1995). https://doi.org/10.1007/BF00866924

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Key words

  • Gliomas
  • Meningiomas
  • Mutations pattern
  • Allele loss