Pediatric Nephrology

, Volume 7, Issue 4, pp 424–427 | Cite as

Hepatitis C infection in children and adolescents on haemodialysis and after renal transplant

  • M. Greco
  • K. Cristiano
  • G. Leozappa
  • M. Rapicetta
  • G. Rizzoni
Original Article

Abstract

Serum antibodies to hepatitis C virus (HCV) were measured in children and adolescents on haemodialysis (HD,n=20) and after renal transplant (RT,n=33). Seropositivity was observed in 3 HD patients (15%) and in 7 RT patients (21.2%) with an enzyme-linked immunosorbent assay (2nd generation) and a recombinant immunoblotting assay (2nd generation). HCV RNA was detected by the polymerase chain reaction in the 10 patients with anti-HCV antibodies. Anti-HCV positivity was significantly correlated (P<0.05) with the number of blood transfusions and the time on HD. Transaminase levels were not useful for screening. This study confirms that there is a high risk of HCV infection in children and adolescents on HD or after RT. Moreover, HCV infection is closely related to the number of blood transfusions as well as the time on HD.

Key words

Hepatitis C virus Haemodialysis Renal transplantation Blood transfusions 

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References

  1. 1.
    Harmon WE (1991) Opportunistic infections in children following renal transplantation. Pediatr Nephrol 5: 118–125Google Scholar
  2. 2.
    Fenell RS, Andres JM, Pfaff W, Richard GA (1981) Liver dysfunction in children and adolescents during hemodialysis and after renal transplantation. Pediatrics 67: 855–861Google Scholar
  3. 3.
    Ruiz P, Gomez F, Schreiber A (1990) Impaired function of macrophage Fc γ receptors in end-stage renal disease. N Engl J Med 322: 717–722Google Scholar
  4. 4.
    Tolkoff-Rubin N, Rubin R (1990) Uremia and host defense. N Engl J Med 322: 770–772Google Scholar
  5. 5.
    Fassbinder W, Brunner FP, Brynger H, Ehrich JHH, Geerlings W, Raine AEG, Rizzoni G, Selwood NH, Tufveson G, Wing AJ (1991) Combined report on regular dialysis and transplantation in Europe, XX, 1989. Nephrol Dial Transplant 6 [Suppl 1]Google Scholar
  6. 6.
    Seaworth BJ, Garrett LE, Stead WW, Hamilton JD (1984) Non-A, non-B hepatitis and chronic dialysis — another dilemma. Am J Nephrol 4: 235–239Google Scholar
  7. 7.
    Kuo G, Choo QL, Alter HJ, Gitnick GL, Redeker AG, Purcell RH, Miyamura T, Dienstag JL, Alter MJ, Stevens CE, Tegtmeier GE, Bonino F, Colombo M, Lee WS, Kuo C, Berger K, Shuster JR, Overby LR, Bradley DW, Houghton M (1989) An assay for circulating antibodies to a major aetiologic virus of human non-A, non-B hepatitis. Science 244: 362–364Google Scholar
  8. 8.
    Choo QL, Kuo G, Weiner AJ, Overby LR, Bradley DW, Honghton M (1989) Isolation of a cDNA clone derived from a blood borne non-A, non-B viral hepatitis genome. Science 244: 359–362Google Scholar
  9. 9.
    Weiner AJ, Kuo G, Bradley DW, Bonino F, Saracco G, Lee C, Rosenblat J, Choo QL, Houghton M (1990) Detection of hepatitis C viral sequences in non-A, non-B hepatitis. Lancet 335: 1–3Google Scholar
  10. 10.
    Ponz E, Campistol JM, Barrera JM, Gil C, Pinto J, Andreu J, Bruguera M (1991) Hepatitis C virus antibodies in patients on hemodialysis and after kidney transplantation. Transplantation Proc 23: 1371–1372Google Scholar
  11. 11.
    Kallinowski B, Theilmann L, Gmelin K, Andrassy K, Deppermann D, Weinel B, Kommerell B (1991) Prevalence of antibodies to hepatitis C virus in hemodialysis patients. Nephron 59: 236–238Google Scholar
  12. 12.
    Alonso MC, Novoa D, Romero R, Arcocha V, Sanchez-Guisande D (1991) Antibodies to hepatitis C virus in patients on haemodialysis. Nephron 57: 247Google Scholar
  13. 13.
    Almoroth G, Ekermo B, Franzen L, Hed J (1991) Antibody responses to hepatitis C virus and its modes of transmission in dialysis patients. Nephron 59: 232–235Google Scholar
  14. 14.
    Roger SD, Crewe E, Cunningham A, Harris DCH (1991) Hepatitis C virus infection in haemodialysis patients. Aust NZ J Med 21: 22–24Google Scholar
  15. 15.
    Fabrizi F, Di Filippo S, Erba G, Bacchini G, Marcelli D, Pontoriero G, Crepaldi M, Locatelli F (1992) Hepatitis C virus infection and hepatic function in chronic hemodialysis patients. Nephron 61: 119Google Scholar
  16. 16.
    Zeldis JB, Depner TA, Kuramoto IK, Gish RG, Holland PV (1990) The prevalence of hepatitis C virus antibodies among hemodialysis patients. Ann Intern Med 112: 958–960Google Scholar
  17. 17.
    Di Maggio A, Montemurro NE, Marangi AL, Montanaro A, De Siati M Ariano V, Coviello F, Godino F, Orbello G, Basile C, Scatizzi A (1991) Prevalence of hepatitis C antibodies in hemodialysis patients and staff. A preliminary study. J Nephrol 2: 83–87Google Scholar
  18. 18.
    Ponz E, Campistol JM, Bruguera M, Barrera JM, Gil C, Pinto JB, Andreu J (1991) Hepatis C virus infection among kidney transplant recipients. Kidney Int 40: 748–751Google Scholar
  19. 19.
    Machida J, Yamaguchi K, Ueda S, Yoshida M, Kusumoto Y, Nishimura Y, Futami G, Ishii T, Watanabe T, Takatsuki K (1992) High incidence of hepatitis C virus antibodies in hemodialysis patients. Nephron 60: 117–118Google Scholar
  20. 20.
    Muller GY, Zabaleta ME, Arminio A, Colmenares CJ, Capriles FI, Bianco NE Machado IV (1992) Risk factors for dialysis-associated hepatitis C in Venuezela. Kidney Int 41: 1055–1058Google Scholar
  21. 21.
    Alfurayh O, Sobh M, Buali AR, Ashraf Ali M, Barri Y, Qunibi W, Taher S (1992) Hepatitis C virus infection in chronic haemodialysis patients, a clinicopathologic study. Nephrol Dial Transplant 7: 327–332Google Scholar
  22. 22.
    Elisaf M, Tsianos E, Mavridis A, Dardamanis M, Pappas M, Siamopoulos KC (1991) Antibodies against hepatitis C virus (anti-HCV) in haemodialysis patients: association with hepatitis B serological markers”. Nephrol Dial Transplant 6: 476–479Google Scholar
  23. 23.
    Chan TM, Lok ASF, Cheng IKP (1991) Hepatitis C infection among dialysis patients: a comparison between patients on maintenance haemodialysis and continuous ambulatory peritoneal dialysis. Nephrol Dial Transplant 6: 944–947Google Scholar
  24. 24.
    Lin HH, Huang CC, Sheen IS, Lin DY, Liaw YF (1991) Prevalence of antibodies to hepatitis C virus in the hemodialysis unit. Am J Nephrol 11: 192–194Google Scholar
  25. 25.
    Jonas MM, Zilleruelo GE, LaRue SI, Abitbol C, Strauss J, Lu Y (1991) Hepatitis C infection in a pediatric dialysis population Pediatrics 89: 707–709Google Scholar
  26. 26.
    Cristiano K, Di Bisceglie AM, Hoofnagle JH, Feinstone SM (1991) Hepatitis C viral RNA in serum of patients with chronic non-A non-B hepatitis: detection by the polymerase chain reaction using multiple primer sets. Hepatology 14: 51–55Google Scholar
  27. 27.
    Rapicetta M, Attili AF, Mele A, De Santis A, Chionne P, Cristiano K, Spada E, Giuliani E, Carli L, Goffredo F, Capoccia L (1992) Prevalence of hepatitis C virus antibodies and hepatitis C virus-RNA in an urban population. J Med Virol 37: 87–92Google Scholar
  28. 28.
    Geerlings W, Brunner FP, Ehrich JHH, Fassbinder W, Landais P, Mallick N, Margreiter R, Raine AEG, Rizzoni G, Selwood NH, Tufveson G (1991) Combined report on regular dialysis and transplantation in Europe, XXI, 1990. 1. Nephrol Dial Transplant 6: 751–840Google Scholar
  29. 29.
    Pereira BJG, Milford EL, Kirkman RL, Levey A (1991) Transmission of hepatitis C virus by orgn transplantation. N Engl J Med 325: 454–460Google Scholar
  30. 30.
    Perez-Fontan M, Moncalian J, Rodriguez-Carmona A, Arrojo F (1991) Prevalence of antihepatitis C antibodies in patients treated with continuous ambulatory peritoneal dialysis and haemodialysis. Nephron 58: 381–382Google Scholar
  31. 31.
    Moroni GA, Cori P, Marelli F, Del Prete M, Padovese P, Gallieni M, Anelli A, Brancaccio D (1991) Indirect evidence for transfusion role in conditioning hepatitis C virus prevalence among dialysis patients. Nephron 57: 371–372Google Scholar
  32. 32.
    Busch MP, Wilber JC (1992) Hepatitis C virus replication. N Engl J Med 326: 64–65Google Scholar
  33. 33.
    Kiyosawa H, Sodeyama T, Tanaka E, Nakano Y, Furuta S, Nishioka K, Purcell RH, Alter HJ (1991) Hepatitis C in hospital employees with needlestick injuries. Ann Intern Med 115: 367–369Google Scholar
  34. 34.
    Katkov WN, Dienstag JL, Cody H, Evans AA, Choo QL, Houghton M, Kuo G (1991) Role of hepatitis C virus in non-B chronic liver disease. Arch Intern Med 151: 1548–1552Google Scholar
  35. 35.
    Alberti A, Morsica G, Chemello L, Cavalletto D, Noventa F, Pontisso P, Roul A (1992) Hepatitis C viraemia and liver disease in symptom-free individuals with anti-HCV. Lancet 340: 697–698Google Scholar
  36. 36.
    Bruix J, Barrera JM, Calvet X, Ercilla G, Costa J, Sanchez-Taplas JM, Ventura M, Vall M, Bruguera M, Bru C, Castillo R, Rodes J (1989) Prevalence of antibodies to hepatitis C virus in Spanish patients with hepatocellular carcinoma and hepatic cirrhosis. Lancet II: 1004–1006Google Scholar
  37. 37.
    Colombo M, Kuo G, Choo QL, Donato MF, Del Ninno E, Tommasini MA, Dioguardi N, Houghton M (1989) Prevalence of antibodies to hepatitis C virus in Italian patients with hepatocellular carcinoma. Lancet II: 1006–1008Google Scholar
  38. 38.
    Shindo M, Di Bisceglie A, Cheung L, Shih WK, Cristiano K, Feinstone SM, Hoofnagle JH (1991) Decrease in serum hepatitis C viral RNA during alpha-interferon therapy for chronic hepatitis C. Ann Intern Med 115: 700–704Google Scholar
  39. 39.
    Shindo M, Di Bisceglie AM, Hoofnagle JH (1992) Long-term follow-up of patients with chronic hepatitis C treated with alfa-interferon. Hepatology 15: 1013–1016Google Scholar
  40. 40.
    Wright HI, Gavaler JS, Van Thiel DH (1992) Preliminary experience with alpha-2-b-interferon therapy of viral hepatitis in liver allograft recipients. Transplantation 53: 121–124Google Scholar

Copyright information

© IPNA 1993

Authors and Affiliations

  • M. Greco
    • 1
  • K. Cristiano
    • 2
  • G. Leozappa
    • 1
  • M. Rapicetta
    • 2
  • G. Rizzoni
    • 1
  1. 1.Division of Nephrology and DialysisBambino Gesu Children's Research HospitalRomeItaly
  2. 2.Department of VirologyIstituto Superiore di SanitàRomeItaly

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