Exercise-induced changes in the expression of surface adhesion molecules on circulating granulocytes and lymphocytes subpopulations

  • Y. Kurokawa
  • S. Shinkai
  • J. Torii
  • S. Hino
  • P. N. Shek
Original Article
  • 96 Downloads

Abstract

This study examined the relationship between exercise-induced changes in the concentration of circulating immunocompetent cells and their surface expression of adhesion molecules: L-selectin (CD62L) and threeβ2-integrins [LFA-1(CD11a/CD18), Mac-1 (CD11b/CD18), and p150/95(CD11c/CD18)]. Eight young male volunteers exercised on a cycle ergometer for 60 min at 60% maximal oxygen uptake. Peripheral blood samples, collected every 30 min throughout exercise and during the 2-h recovery period, were used for flow-cytometric analysis. The experimental results were compared with control data obtained ever 60 min at corresponding times of the nonexercise day. The exercise regimen induced a granulocytosis and a lymphocytosis, mainly due to an elevation of CD8+ and CD16+ cells. During recovery, a further granulocytosis occurred but accompanied by a lymphopenia. The increased CD8+ cell-count during exercise was characterized by a selective mobilization of the CD62L and CD11ahigh cells, i.e.primed CD8+ cells. A postexercise suppression of CD4+ cell-count was derived only from CD62L+ cells. The CD11b+ and CD11c+ lymphocytes also increased during exercise, largely attributable to an increase in CD16+ cells which co-expressed CD11b and CD11c molecules. The CD62L surface density of granulocytes increased significantly during recovery. This resulted from a selective influx of CD62Lhigh granulocytes into the circulation. There were no significant changes in per-cell density of the threeβ2-integrins on granulocytes and lymphocytes throughout the experimental period. These results suggest that the cell-surface expression of CD62L (and CD I la) molecules is associated with the differential mobilization of CD8+ cells during exercise, the postexercise suppression of CD4 cell-counts and the granulocytosis following exercise.

Key words

Physical exercise Leukocyte Adhesion molecule L-selectin β2-integrin 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Y. Kurokawa
    • 1
  • S. Shinkai
    • 1
  • J. Torii
    • 2
  • S. Hino
    • 3
  • P. N. Shek
    • 4
    • 5
  1. 1.Departments of Hygiene and Public HealthEhime University School of MedicineEhimeJapan
  2. 2.Department of Medical Laboratory TechnologyEhime College of Health ScienceEhimeJapan
  3. 3.Department of NutritiopnYamaguchi Women's CollegeYamaguchiJapan
  4. 4.Operation Medicine DivisionDefence and Civil Institute of Environmental MedicineNorth YorkCanada
  5. 5.Department of Clinical Biochemistry, Faculty of MedicineUniversity of TorontoTorontoCanada

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