Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Transcription during early embryogenesis ofLeptinotarsa (Coleoptera)

An autoradiographic analysis

  • 34 Accesses

  • 5 Citations


RNA synthesis was investigated in precleavage stages (0–5 h after egg deposition) and cleavage stages (5–36 h) ofLeptinotarsa by autoradiography. In precleavage stages the first detectable RNA synthesis occurs after the maturation divisions. Label is found in the female pronucleus during the short period of migration towards the interior of the egg and in the polar body nuclei. In no case was label found in the sperm pronuclei or during the period when the female pronucleus is attached to a male pronucleus prior to fusion (pronuclear prefusion phase). During cleavage, intranuclear labelling can be shown at all developmental stages except the 2-nuclei stage. RNA synthesis increases with age. α-Amanitin completely inhibits the autoradiographically detectable RNA synthesis.

This is a preview of subscription content, log in to check access.


  1. Bier, K.: Autoradiographische Untersuchungen über die Leistungen des Follikelepithels und der Nährzellen bei der Dotterbildung und Eiweißsynthese im Fliegenovar. Wilhelm Roux' Archiv154, 552–575 (1963)

  2. Brown, D.D.: RNA synthesis during Amphibian development. J. Exp. Zool.157, 101–113 (1964)

  3. Büning, J.: Changes in RNA synthesis of endogenous “free” and template-engaged RNA polymerase in isolated nuclei of highly synchronized coleopteran embryos. Dev. Biol.64, 130–139 (1978)

  4. Davidson, E.H.: Gene activity in early development. Second edition. New York, San Francisco, London: Academic Press 1976

  5. Doenecke, D., Pfeiffer, CH., Sekeris, C.E.: Multiple forms of DNA-dependent RNA polymerase from insect tissue FEBS Lett.21, 2, 237–243 (1972)

  6. Duspiva, F., Scheller, K., Weiß, D., Winter, H.: Ribonucleinsäuresynthese in der telotroph-meroistischen Ovariole vonDysdercus intermedius Dist (Heteroptera, Pyrrhoc.). Wilhelm Roux' Archiv172, 83–130 (1973)

  7. Edenberg, H.J., Huberman, J.A.: Eukaryotik chromosome replication. Ann. Rev. Gen.9, 245–284 (1975)

  8. Eudy, W.W., Dobrogosz, W.J.: Radioisotope incorporation and nucleic acids synthesis in dipteran embryos. J. Cell Biol.35, 37A (1967)

  9. Fausto-Sterling, A., Zheutlin, L.M., Brown, P.R.: Rates of RNA synthesis during early embryogenesis inDrosophila melanogaster. Dev. Biol.40, 78–83 (1974)

  10. Greenleaf, A.F., Bautz, K.F.: RNA polymerase B fromDrosophila melanogaster larvae. Eur. J. Biochem.60, 169–179 (1975)

  11. Gurdon, J.B., Woodland, H.R.: The influence of the cytoplasm on the nucleus during cell differentiation, with special reference to RNA synthesis during amphibian cleavage. Proc. Roy. Soc. B.173, 99–111 (1969)

  12. Haget, M.A.: Analyse experimentale des facteurs de la morphogénèse embryonnaire chez le coléoptèreLeptinotarsa. Bull Biol. Fr. Belg.87, 123 (1953)

  13. Haget, M.A.: Quelques données sur la maturation et l'activation de l'oeuf deDoryphore (Leptinotarsa decemlineata Say.). C.R. Acad. Sci. (Paris)269, 2128–2131 (1969)

  14. Hansen-Delkeskamp, E., Sauer, H.W., Duspiva, F.: Ribonucleinsäuren in der Embryogenese vonAcheta domestica. Naturforsch.22b, 540–545 (1967)

  15. Harris, S.E., Forrest, H.S.: RNA polymerase activity in isolated nuclei from eggs ofOncopeltus fasciatus. J. Insect Physiol.17, 303–312 (1971)

  16. Lamb, M.M., Laird, CH.D.: Increase in nuclear poly(A) containing RNA at syncytial blastoderm inDrosophila melanogaster embryos. Dev. Biol.52, 31–42 (1976)

  17. Lockshin, R.A.: Insect embryogenesis: Macromolecular synthesis during early development. Science154, 775–776 (1966)

  18. Maisonhaute, C.: Observations cytologiques et autoradiographiques sur les premiers stades embryonnaires duDoryphore (Leptinotarsa decemlineata Say). C.R. Acad. Sci. (Paris)272, 1398–1401 (1971)

  19. Maisonhaute, C.: Comparaison des effects de deux inhibiteurs de la synthèse d'ARN (actinomycin D et α-amanitine) sur le développement embryonnaire d'un insecte: déterminisme génique de début de l'embryogénèse deLeptinotarsa dec. Wilhelm Roux's Archives183, 61–77 (1977)

  20. Mintz, B.: Synthetic processes and early development in the mammalian egg. J. Exp. Zool.157, 85–100 (1964)

  21. Pietruschka, F., Bier, K.-H.: Autoradiographische Untersuchungen zur RNA- und Proteinsynthese in der frühen Embryogenese vonMusca domestica. Wilhelm Roux' Archiv169, 56–69 (1972)

  22. Rinaldi, A.M., Monroy, A.: Polyribosome formation and RNA synthesis in the early post-fertilization stages of the sea urchin egg. Dev. Biol.19, 73–86 (1969)

  23. Scheller, K., Karlson, P.: Effects of ecdysteroids on RNA synthesis of fat body cells inCalliphora vicina. J. Insect. Physiol.23, 285–291 (1977)

  24. Scheller, K., Karlson, P., Bodenstein, D.: Effects of ecdysterone and the juvenile hormone analogueMethoprene on protein, RNA and DNA synthesis in wing discs ofCalliphora vicina. Naturforsch.33c, 253–260 (1978)

  25. Schenkel, H.: Autoradiographische Untersuchung der RNA- und DNA-Synthese in der frühen Embryonalentwicklung vonLeptinotarsa dec. Staatsexamensarbeit. Zool. Inst. Heidelberg (1973)

  26. Schmidt, O., Jäckle, H.: RNA synthesised during oogenesis and early embryogenesis in a insect egg (Euscelis plebejus). Wilhelm Roux' Archiv184, 143–153 (1978)

  27. Schnetter, W.: Experimente zur Analyse der morphogenetischen Funktion der Ooplasmabestandteile in der Embryonalentwicklung desKartoffelkäfers (Lept. dec.). Wilhelm Roux' Archiv155, 637–692 (1965)

  28. Schnetter, W.: Zur Proteinsynthese in der frühen Embryonalentwicklung vonLeptinotarsa dec. (Coleoptera): Rolle der mütterlichen und der embryoeigenen Messenger-RNA. Verh. Dtsch. Zool. Ges. 197–201 (1975)

  29. Scriba, M.E.L.: Embryonale Entwicklungsstörungen bei Nullosomie und Tetrasomie des 3. Chromosoms vonDrosophila melanogaster. Dev. Biol.11, 160–177 (1969)

  30. Wilt, F.H.: The acceleration of ribonucleic acid synthesis in cleaving sea urchin embryos. Dev. Biol.23, 444–455 (1970)

  31. Winter, H.: Charakteristika mütterlicher RNP-Partikel aus dem Ei der Baumwollwanze,Dysdercus intermedius Dist. Verh. Dtsch. Zool. Ges.1974, 201–204 (1975)

  32. Winter, H., Wiemann-Weiss, D., Duspiva, F.: Endogene Synthese kurzlebiger Messenger-RNA in der Oocyte vonDysdercus intermedius Dist. nach Abschluß der Vitellogenese. Wilhelm Roux's Archives182, 39–58 (1977)

  33. Zalokar, M.: Autoradiographic study of protein und RNA formation during early development ofDrosophila eggs. Dev. Biol.49, 425–437 (1976)

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Schenkel, H., Schnetter, W. Transcription during early embryogenesis ofLeptinotarsa (Coleoptera). Wilhelm Roux' Archiv 186, 179–188 (1979). https://doi.org/10.1007/BF00848588

Download citation

Key words

  • Insects
  • Early embryogenesis
  • RNA synthesis
  • α-amanitin