Stage specific synthesis of some follicle cell proteins inDrosophila melanogaster

  • Katrin Lineruth
  • Andrew Lambertsson
Rapid Communications
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Accepted:

Summary

Ovarian protein synthesis in the temperature-sensitive mutantl(1)su(f)ts67g was analysed at the permissive and non-permissive temperature by SDS-polyacrylamide gel electrophoresis of35S-methionine labelled ovaries. The synthesis of yolk and three other ovarian proteins of approximative molecular weights of 92K, 82K and 76K, respectively, were affected by the shift to the restrictive temperature. Examination of protein synthesis pattern in staged egg chambers revealed that these three proteins were synthesized at stage 10. Analysis of separated cell types present at stage 10 demonstrate that the three proteins were follicle cell products. We have been unable to identify these proteins as any previously described follicle cell proteins.

Key words

Drosophila oogenesis Protein synthesis SDS-gel electrophoresis Temperature-sensitive mutant 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Katrin Lineruth
    • 1
  • Andrew Lambertsson
    • 1
  1. 1.Department of GeneticsUniversity of UmeåUmeåSweden

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