In vitro study of selective endocytosis of vitellogenin by locust oocytes

  • Axel Röhrkasten
  • Hans-Jörg Ferenz


Oocytes ofLocusta migratoria selectively sequester the female-specific protein vitellogenin at a high rate. In vitro studies using in vivo radiolabelled vitellogenin show that uptake rates increase proportionally with time and temperature (with a sharp change between 10° and 15°C). They also depend on oocyte size and vitellogenin concentration. The uptake process is saturable. Vitellogenin is taken up more specifically than vitellin. Bovine serum albumin, casein and male locust haemolymph proteins do not inhibit endocytosis of vitellogenin. However, several metabolic inhibitors and phosphorylated compounds, such as phosvitin or phosphohexoses, are potent inhibitors of vitelogenin incorporation.

Key words

Insects Locusta Oocytes Vitellogenin Endocytosis 


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Axel Röhrkasten
    • 1
  • Hans-Jörg Ferenz
    • 1
  1. 1.Fachbereich BiologieUniversität OldenburgOldenburgFederal Republic of Germany

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