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Areal and laminar distribution of gangliosides in the fetal human neopallium at 28 weeks of gestation

  • I. Kraêun
  • H. Rösner
  • I. Kostoviê
  • H. Rahmann
Rapid Communications

Summary

Correlative histological, histochemical and biochemical investigations on laminar compartments from four different areas of fetal human neopallium at 28 weeks of gestation revealed discrete distribution of gangliosides in the cerebral wall. Highest level of total ganglioside concentration was found in the layers of cortical anlage (cortical plate and “subplate layer”) which are concomittantly characterized by highest activity of acetylcholinesterase (AChE) and which are known to be involved in intensive synaptogenesis at this stage of cortical development. In three of four areas the proportion of GD1a — ganglioside from total ganglioside amount tended to increase and that of GT1b to decrease from inside (ventricle) to outside (cortical anlage) throughout the cerebral wall.

Key-words

Gangliosides Fetal human brain Acetylcholinesterase 

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References

  1. Barondes SH (1970) Brain Glycomacromolecules and interneuronal recognition. In: The neurosciences: second study program. Schmitt FO (ed) Rockefeller University Press, NY, pp 747–760Google Scholar
  2. Brückner G, Braulke T, Müller L, Biesold D (1982) Dissection of embryonic rat cerebral cortex into individual layers: biochemical studies on glycoproteins and related enzymes. Neurosci. Suppl vol. 7: Abstract S 33Google Scholar
  3. Derry DM, Wolfe LS (1967) Gangliosides in isolated neurons and glial cells. Science 158:1450PubMedGoogle Scholar
  4. Dreyfus H, Urban PF, Edel-Harth S, Mandel P (1975) Developmental patterns of gangliosides and of phospholipids in chick retina and brain. J Neurochem 25:245–250PubMedGoogle Scholar
  5. Hansson HH, Holmgren J, Svennerholm L (1977) Ultrastructural localization of cell membrane GM1 ganglioside by cholera toxin. Proc Natl Acad Sci USA 74:3782–3786PubMedGoogle Scholar
  6. Irwin LN, Irwin CC (1979) Developmental changes in ganglioside composition of hippocampus, retina and optic tectum. Dev Neurosci 2:129–157Google Scholar
  7. Jacobovitz DM (1974) Removal of discrete fresh regions of the rat brain. Brain Res 80:111–115PubMedGoogle Scholar
  8. Jacobsen M (1978) Developmental neurobiology. Plenum Press NYGoogle Scholar
  9. Kostović (1979) Columnar distribution of acetylcholinesterase staining in the frontal cortex of the human fetus. Neurosci Lett, Supp 3:S22, abstractGoogle Scholar
  10. Kostović I, Krmpotić-Nemanić J (1976) Early prenatal ontogenesis of the neuronal connections in the interhemispheric cortex of the human gyrus cinguli. Verh Anat Ges 70:305–316PubMedGoogle Scholar
  11. Kostović I, Molliver MW (1974) A new interpretation of the laminar development of cerebral cortex: synaptogenesis in different layers of the neopallium in the human fetus. Anat Res 178:395Google Scholar
  12. Kostović I, Krmpotić-Nemanić J, Kelović Z, Kracum I (1979) The development of “cholinergic” fibres in different cytoarchitectonic compartments of neocortex in the human fetus. Anat Res 193:591–592Google Scholar
  13. Kostović-Knezević LJ, Kostović I, Krmpotić-Nemanić J, Kelović Z (1979) Acetylcholinesterase (AChE) staining in the growing telencephalic structures of the human fetus. Verh Anat Ges 73:667–669Google Scholar
  14. Kracun I, Kostović I, Kelović Z, Krmpotić-Nemanić J,(1980) Histochemical mapping as a tool for biochemical analysis of the developing cellular compartments in the human brain. Proceed 3 rd Mett Europ Soc Neurochem, Bled:592Google Scholar
  15. Krnjević K, Silver A (1966) Acetylcholinesterase in the developing forebrain. J Anat 100:63–89PubMedGoogle Scholar
  16. Ledeen RW (1978) Ganglioside structures and distribution: Are they located at nerve ending? J Supramol Struct 8:1–17PubMedGoogle Scholar
  17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent J Biol Chem 193:265–275PubMedGoogle Scholar
  18. Miettinen T, Takki-Lukkainen IT (1959) Use of butylacetate in determination of sialic acid. Acta Chem Scand 13:856–858Google Scholar
  19. Molliver MW, Kostović I, Van der Loos H (1973) The development of synapses in cerebral cortex of the human fetus. Brain Res 50:403–407PubMedGoogle Scholar
  20. Morgan IG, Zanetta JP, Breckenridge WC, Vincendon G, Gombos G (1973) The chemical structures of synaptic membranes. Brain Res 62:405–411PubMedGoogle Scholar
  21. Moscona AA (1975) Surface specification of embryonic cells: lectin receptor cell recognition, and specific cell ligands. In: Moscona AA (ed) The cell surface in development. Raven Press, New York pp 67–99Google Scholar
  22. Pfenninger KH, Pfenninger MF-M (1979) Surface glycoconjugates in the differentiating neuron. In: Margolis RU, Margolis RK (eds) Complex carbohydrates of nervous tissue. Plenum Press, New York, 185–191Google Scholar
  23. Pfenninger KH, Ress RP (1976) From the growth cone to the synapse. In: Barondes SH (ed) Neuronal recognition. Plenum Press, New York, pp 131–178Google Scholar
  24. Purpura DP (1975) Dendritic differentiation in human cerebral cortex: normal and aberrant developmental patterns. Adv Neurol 12:92–116Google Scholar
  25. Rakić P (1972) Mode of cell migration to the superficial layers of the fetal monkey neocortex. J Comp Neurol 145:61–84PubMedGoogle Scholar
  26. Rösner H (1977) Gangliosides, sialoglycoproteins and AChE of the developing mouse brain. Wilhelm Roux's Arch 183:325–335Google Scholar
  27. Rösner H (1980) Ganglioside changes in the chicken optic lobes and cerebrum during embryonic development Wilhelm Roux's Arch 188:205–213Google Scholar
  28. Rösner H (1982) Ganglioside changes in the chicken optic lobes as biochemical indicators of brain development and maturation. Brain Res 236:46–61Google Scholar
  29. Rösner H (1981) Isolation and preliminary characterisation of novel polysialogangliosides from embryonic chicken brain. J Neurochem 37:993–997PubMedGoogle Scholar
  30. Sidman RL, Rakić P (1973) Neuronal migration, with special references to developing human brain: A review. Brain Res 62:1–35PubMedGoogle Scholar
  31. Sperry RW (1963) Chemoaffinity in the orderly growth of nerve fibre patterns and connection. Proc Natl Acad Sci 50:703–710PubMedGoogle Scholar
  32. Suzuki K (1965) The pattern of mammalian brain gangliosides. III. Regional and developmental differences. J. Neurochem 12:969–979PubMedGoogle Scholar
  33. Svennerholm L (1957) Quantitative estimation of sialic acid. Biochim Biophys Acta 24:604–611PubMedGoogle Scholar
  34. Svennerholm L, Fredman P (1979) A Procedure for the quantitative isolation of brain gangliosides. Biochim Biophys Acta 617:97–109Google Scholar
  35. Vanier MT, Holm M, Öhmann R, Svennerholm L (1971) Developmental profiles of gangliosides in human and rat brain. J Neurochem 18:581–592PubMedGoogle Scholar
  36. Wiegandt H (1967) The subcellular localization of gangliosides in the brain. J Neurochem 14:671–674PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • I. Kraêun
    • 1
  • H. Rösner
    • 3
  • I. Kostoviê
    • 2
  • H. Rahmann
    • 3
  1. 1.Department of BiochemistryUniversity of ZagrebZagrebYugoslavia
  2. 2.Department of AnatomyUniversity of ZagrebZagrebYugoslavia
  3. 3.Institut für Zoologie der Universität Stuttgart-HohenheimStuttgart 70Federal Republic of Germany

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