Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Aggregative properties of different cell types of the fresh-water spongeEphydatia fluviatilis isolated on ficoll gradients


Cell suspensions of the fresh-watersponge Ephydatia fluviatilis have been fractionated by means ofFicoll gradient centrifugation. Three fractions were isolated. The densest contains archeocyte-like cells only; the intermediate fraction is very rich in choanocytes, and the lightest is a mixture of cell types. Earch fraction shows specificaggregative properties and potentialities to reconstitute functional sponges.

It appears that the sequence of reconstitution events can be selectively altered by certain disequilibria in the cell population

These preliminary results constitute a first approach to the analysis ofcell type specificity in sponges.

This is a preview of subscription content, log in to check access.

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.


  1. Borojevic, R.: Essai de reconstitution du choanoderme à partir d'une suspension choanocytaire d'Eponge acalcaire. Comptes rendus de l'Académie des Sciences de Paris257, 961–962 (1963)

  2. Brien, P.: La réorganisation de l'éponge après dissociation.Ephydatia fluviatilis (L). Arch. de Biol.48, 185 (1937)

  3. Curtis, A.S.G., Van De Vyver, G.: The control of cell adhesion in a morphogenetic system. J. of Embryol. and Exp. morphology,26, 245–312 (1971)

  4. Giudice, G.: The mechanism of aggregation of embryonic sea urchin cells; a biological approach. Developmental Biology, 12 p. 233–247 (1965)

  5. Humphreys, T.: Aggregation of chemically dissociated sponge cells in the absence of protein synthesis. Journal of Experimental Zoology,160, 235–240 (1965)

  6. John, H.A., Campo, M.S., Mackenzie, A.M., Kemp, R.B.: Role of different sponge cell types in species specific cell aggregation. Nature New Biology,12, 126–128 (1971)

  7. Leith, A.G., Steinberg, M.S.: Sponge cell adhesion. Velocity sedimentation, separation and aggregative specificity of discrete cell types. Biological bulletin,143, 468–472 (1972)

  8. Moscona, M.H., Moscona, A.A.: Inhibition of adhesiveness and aggregation of dissociated cells by inhibitors of protein and RNA synthesis. Science142, 1070–1071 (1963)

  9. Pessac, B., Defendi, V.: Evidence for distinct aggregation factors and receptors in cells. Nature New Biology,238, 13–15 (1972)

  10. Rasmont, R.: Une technique de culture des Eponges d'eau douce en milieu contrôlé. Annales de la Soc. roy. zool. de Belgique,91, 147–156 (1961)

  11. Turner, R.S., Burger, M.: Involvement of a carbohydrate group in the active site for surface guided reassociation of animal cells. Nature,244, 509–510 (1973)

  12. Van de Vyver, G.: Mise en évidence d'un facteur d'agrégation chez l'Eponge d'eau douceEphydatia fluviatilis. Annales d'embryologie et de morphogénèse,4 (4), 373–381 (1971)

  13. Van de Vyver, G.: Phenomena of cellular recognition in sponges. Current topics in Developmental biology,10, 123–140 (1975)

  14. Weinbaum, G., Burger, M.M.: Two component system for surface guided reassociation of animal cells. Nature,244, 510–512 (1973)

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

De Sutter, D., Van de Vyver, G. Aggregative properties of different cell types of the fresh-water spongeEphydatia fluviatilis isolated on ficoll gradients. Wilhelm Roux' Archiv 181, 151–161 (1977).

Download citation

Key words

  • Sponge
  • Ficoll gradient
  • Aggregative Properties
  • Cell type specificity