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Aggregative properties of different cell types of the fresh-water spongeEphydatia fluviatilis isolated on ficoll gradients

Summary

Cell suspensions of the fresh-watersponge Ephydatia fluviatilis have been fractionated by means ofFicoll gradient centrifugation. Three fractions were isolated. The densest contains archeocyte-like cells only; the intermediate fraction is very rich in choanocytes, and the lightest is a mixture of cell types. Earch fraction shows specificaggregative properties and potentialities to reconstitute functional sponges.

It appears that the sequence of reconstitution events can be selectively altered by certain disequilibria in the cell population

These preliminary results constitute a first approach to the analysis ofcell type specificity in sponges.

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De Sutter, D., Van de Vyver, G. Aggregative properties of different cell types of the fresh-water spongeEphydatia fluviatilis isolated on ficoll gradients. Wilhelm Roux' Archiv 181, 151–161 (1977). https://doi.org/10.1007/BF00848439

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Key words

  • Sponge
  • Ficoll gradient
  • Aggregative Properties
  • Cell type specificity