Wilhelm Roux's archives of developmental biology

, Volume 194, Issue 2, pp 79–86 | Cite as

Head regeneration and polarity reversal inHydra attenuata can occur in the absence of DNA synthesis

  • Susan G. Cummings
  • Hans R. Bode
Article

Summary

Regeneration in hydra is considered to be morphallactic because it can occur in the absence of cell division. Whether DNA synthesis is required for regeneration or other repatterning events is not known. The question was investigated by blocking DNA synthesis with hydroxyurea and examining several developmental processes. Head regeneration, reversal of regeneration polarity and battery cell differentiation all took place in the absence of DNA synthesis. Hence, morphallactic regulation in hydra is independent of both DNA synthesis and mitosis.

Key words

Hydra Morphallactic regeneration Polarity reversal DNA synthesis Epithelial cells 

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References

  1. Berking S (1979) Control of nerve cell formation from multipotent stem cells in hydra. J Cell Sci 40:198–205Google Scholar
  2. Bode HR, David CN (1978) Regulation of a multipotent stem cell, the interstitial cell of Hydra. Prog Biophys Mol Biol 33:198–206Google Scholar
  3. Bode HR, Flick KM, Smith GS (1976) Regulation of interstitial cell differentiation in Hydra attenuata. I. Homeostatic control of interstitial cell population size. J Cell Sci 20:29–46Google Scholar
  4. Campbell RD (1965) Proliferation in Hydra: An autoradiographic approach. Science 148:1231–1232Google Scholar
  5. Campbell RD (1967) Tissue dynamics of steady state growth in Hydra littoralis. III. Behavior of specific cell types during tissue movements. J Exp Zool 164:379–392Google Scholar
  6. Campbell RD (1973) Vital marking of single cells in developing tissues: India ink injection to trace tissue movements in Hydra. J Cell Sci 13:651–661Google Scholar
  7. Campbell RD, David CN (1974) Cell cycle kinetics and development of Hydra attenuata. II. Interstitial cells. J Cell Sci 16:349–358Google Scholar
  8. Clarkson SG (1969) Nucleic acid and protein synthesis and pattern regulation in hydra. II. Effect of inhibition of nucleic acid and protein synthesis on hypostome formation. J Embryol Exp Morphol 21:55–70Google Scholar
  9. Cooke J (1973) Morphogenesis and regulation in spite of continued mitotic inhibition in Xenopus embryos. Nature 242:55–57Google Scholar
  10. David CN (1973) A quantitative method of maceration of Hydra tissue. Wilhelm Roux's Arch 171:259–268Google Scholar
  11. David CN, Campbell RD (1972) Cell cycle kinetics and development of Hydra attenuata. I. Epithelial cells. J Cell Sci 11:557–568Google Scholar
  12. David CN, Murphy S (1977) Characterization of interstitial stem cells in hydra by cloning. Dev Biol 48:372–383Google Scholar
  13. Diehl FA, Burnett AL (1965) The role of interstitial cells in the maintenance of hydra. III. Regeneration of hypostome and tentacles. J Exp Zool 158:299–318Google Scholar
  14. Fujisawa T, David CN (1981) Commitment during nematocyte differentiation in Hydra. J Cell Sci 48:207–222Google Scholar
  15. Gambari R, Marks P, Rifkind R (1979) Murine erythroleukemia cell differentiation: relationship of globin gene expression and of prolongation of G1 to inducer effects during G1/early S. Proc Natl Acad Sci USA 76:4511–4515Google Scholar
  16. Graham TM (1974) DNA synthesis and effects of hydroxyurea on regenerating Hydra. Trans Ill State Acad Sci 67:222–227Google Scholar
  17. Hicklin J, Wolpert L (1973) Positional information and pattern regulation in Hydra: The effect of radiation. J Embryol Exp Morphol 30:741–752Google Scholar
  18. Humason GL (1962) Animal tissue techniques. Freeman WH and Company, p 569Google Scholar
  19. Jones PA, Taylor SM (1980) Cellular differentiation, cytidine analogs and DNA methylation. Cell 20:85–93Google Scholar
  20. Lesh-Laurie GE, Brooks DC, Kaplan ER (1976) Biosynthetic events of hydroid regeneration. I. The role of DNA synthesis during tentacle elaboration. Wilhelm Roux's Arch 180:157–174Google Scholar
  21. Linkhart T, Clegg C, Hauschka S (1980) Control of mouse myoblast commitment to terminal differentiation by mitogens. In: Cunningham D (eds) ICN/UCLA symposium un control of cell division and differentiation. Keystone, ColoradoGoogle Scholar
  22. Loomis WF, Lenhoff HM (1956) Growth and sexual differentiation of Hydra in mass culture. J Exp Zool 132:555–573Google Scholar
  23. MacWilliams HK (1983) Hydra transplantation phenomena and the mechanism of Hydra head regeneration. II. Properties of head activation. Dev Biol 96:239–257Google Scholar
  24. MacWilliams HK, Bonner JT (1979) The prestalk-prespore pattern in cellular slime molds. Differentiation 14:1–22Google Scholar
  25. Marcum BA, Campbell RD (1978a) Development of hydra lacking nerves and interstitial cells. J Cell Sci 29:17–33Google Scholar
  26. Marcum BA, Campbell RD (1978b) Developmental roles of epithelial cell lineages in Hydra: analysis of chimera. J Cell Sci 32:233–247Google Scholar
  27. Marcum BA, Campbell RD, Romero J (1977) Polarity reversal in nerve-free hydra. Science 197:771–773Google Scholar
  28. Morgan TH (1901) Regeneration in the egg, embryo and adult. Am Natur 35:949–973Google Scholar
  29. Nadal-Ginard B (1978) Commitment, fusion and biochemical differentiation of a myogenic cell line in the absence of DNA synthesis. Cell 15:355–364Google Scholar
  30. Park HD, Ortmeyer AB, Blankenbaker DP (1970) Cell division during regeneration in Hydra. Nature 177:617–619Google Scholar
  31. Razin A, Riggs AD (1980) DNA methylation and gene function. Science 210:604–610Google Scholar
  32. Rubin DI, Bode HR (1982a) The aberrant, a morphological mutant of Hydra attenuata, has altered inhibition properties. Dev Biol 89:316–331Google Scholar
  33. Rubin DI, Bode HR (1982b) Both the epithelial cells and the nerve cells are involved in the head inhibition properties in Hydra attenuata. Dev Biol 89:332–338Google Scholar
  34. Sacks PG, Davis LE (1979) Production of nerveless Hydra attenuata by hydroxyurea treatments. J Cell Sci 37:189–203Google Scholar
  35. Schaller HC, Gierer G (1973) Distribution of the head-activating substance in hydra and its localization in membranous particles in nerve cells. J Embryol Exp Morphol 29:39–52Google Scholar
  36. Smith GH, Vonderhaar BK (1981) Functional differentiation in mouse mammary gland epithelium is attained through DNA synthesis, inconsequent of mitosis. Dev Biol 88:167–179Google Scholar
  37. Takano J, Sugiyama T (1984) Genetic analysis of developmental mechanisms in hydra. XII. Analysis of chimeric hydra produced from a normal and a slow-budding strain (L4). J Embryol Exp Morphol 80:155–173Google Scholar
  38. Venugopal G, David CN (1981) Nerve commitment in Hydra. II. Localization of commitment in S. Dev Biol 83:361–365Google Scholar
  39. Vonderhaar B, Topper Y (1974) A role of the cell cycle in hormone-dependent differentiation. J Cell Biol 63:707–712Google Scholar
  40. Wilby OR, Webster G (1970) Experimental studies on axial polarity in Hydra. J Embryol Exp Morphol 24:595–613Google Scholar
  41. Yaross MS, Baca BA, Chow MH, Bode HR (1982) Commitment of Hydra interstitial cells to nerve cell differentiation occurs by late S-phase. Dev Biol 89:425–436Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Susan G. Cummings
    • 1
  • Hans R. Bode
    • 1
  1. 1.Developmental Biology Center and Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

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