Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Inducing effects of the presumptive endoderm of successive stages inTriturus alpestris

  • 28 Accesses

  • 35 Citations

Summary

  1. 1.

    Using embryos ofTriturus alpestris, a piece of the endoderm of successive stages was explanted together with ectoderm of early gastrulae by the sandwich method.

  2. 2.

    In all series—from the uncleaved egg to the larval stage—endoderm induced mesoderm-derived organs in the ectoderm. Endoderm from uncleaved eggs and 4-cell stages induced only blood and coelomic epithelium, whereas endoderm from the other stages also induced muscle, notochord, and pronephric tubules to a varying degree. Blastula endoderm had the highest inducing activity.

  3. 3.

    Small amounts of intestine were also induced in the ectoderm in some cases. The explanted endoderm itself showed differentiation only when taken from the 8–64 cell stage to early gastrula stage.

Zusammenfassung

  1. 1.

    Entoderm verschiedener Entwicklungsstadien vonTriturus alpestris wurde zusammen mit Ektoderm aus frühen Gastrulastadien nach der Sandwich-Methode explantiert.

  2. 2.

    Entoderm aller Entwicklungsstadien von der ungefurchten Eizelle bis zum Larvenstadium induzierte im Ektoderm Organe, die sich aus dem Mesoderm herleiten. Entoderm aus ungefurchten Eiern und 4-Zell-Stadien induzierte nur Blut und Coelomepithel, während Entoderm aus den anderen Stadien auch Muskel, Chorda und Pronephros induzierte. Blastula-Entoderm hat das höchste Induktionsvermögen.

  3. 3.

    Histologisch erkennbares Darmepithel wurde in geringer Menge ebenfalls induziert. Das explantierte Entoderm selbst zeigte nur Differenzierungen, wenn es aus 8–64 Zellstadien bis zu frühen Gastrulastadien entnommen war.

This is a preview of subscription content, log in to check access.

Literature

  1. Balinsky, B. I.: On the determination of endodermal organs in Amphibia. C. R. Acad. Sci. U.R.S.S.20, 215–217 (1938)

  2. Balinsky, B. I.: Korrelationen in der Entwicklung der Mund- und Kiemenregion und des Darmkanals bei Amphibien. Wilhelm Roux' Arch. Entwickl.-Mech. Org.143, 365–395 (1948)

  3. Boterenbrood, E. C., Nieuwkoop, P. D.: The formation of the mesoderm in urodelean amphibians. V. Its regional induction by the endoderm. Wilhelm Roux' Archiv173, 319–332 (1973)

  4. Holtfreter, J.: Nachweis der Induktionsfähigkeit abgetöteter Keimteile. Isolations- und Transplantationsversuche. Wilhelm Roux' Arch. Entwickl.-Mech. Org.128, 584–633 (1933)

  5. Mangold, O.: Transplantationsversuche zur Frage der Spezifität und der Bildung der Keimblätter. Arch. mikr. Anat.100, 198–301 (1923)

  6. Nakamura, O., Takasaki, H., Yamane, H., Ôbayashi, N., Kôno, S., Okamoto, H., Okumoto, T.: Inductive Activity of the Organizer in the Morula and Blastula ofTriturus pyrrhogaster. Proc. Japan. Acad.46, 700–705 (1970)

  7. Nakamura, O., Takasaki, H., Ishihara, M.: Formation of the Organizer from Combinations of presumptive Ectoderm and Endoderm. I. Proc. Japan. Acad.47, 313–318 (1971)

  8. Niewkoop, P. D., Ubbels, G. A.: The formation of the mesoderm in urodelean Amphibians. IV. Qualitative evidence of the purely “Ectodermal” Origin of the entire mesoderm and of the pharyngeal endoderm. Wilhelm Roux' Archiv169, 185–199 (1972)

  9. Okada, T. S.: Rôle of the mesoderm in the differentiation of endodermal organs. Mem. Coll. Sci. Univ. Kyoto, ser. B.,20, 157–162 (1953a)

  10. Okada, T. S.: Regulative power of the endoderm in Urodele. Zool. Mag., Tokyo,62, 288–291 (1953b)

  11. Okada, T. S.: Experimental studies on the differentiation of the endodermal organs in Amphibia. I. Significance of the mesenchymatous tissue to the differentiation of the presumptive endoderm. Mem. Coll. Sci. Univ. Kyoto, ser. B.21, 1–6 (1954)

  12. Okada, T. S.: Epithelio-mesenchymal relationship in the regional differentiation of the digestive tract in the Amphibian embryo. Wilhelm Roux' Arch. Entwickl.-Mech. Org.152, 1–21 (1960)

  13. Spemann, H., Mangold, H.: Über Induktion von Embryonalanlagen durch Implantation artfremder Organisatoren. Wilhelm Roux' Arch. Entwickl.-Mech. Org.100, 599–638 (1924)

  14. Takata, C.: The differentiationin vitro of the isolated endoderm under the influence of the mesoderm inTriturus pyrrhogaster. Embryologia5, 39–70 (1960a)

  15. Takata, C.: The differentiationin vitro of the isolated endoderm in the presence of the neural fold inTriturus pyrrhogaster. Embryologia5, 194–205 (1960b)

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Asashima, M. Inducing effects of the presumptive endoderm of successive stages inTriturus alpestris . Wilhelm Roux' Archiv 177, 301–308 (1975). https://doi.org/10.1007/BF00848181

Download citation

Keywords

  • Developmental Biology
  • Larval Stage
  • Successive Stage
  • Induce Activity
  • High Induce