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Pediatric Surgery International

, Volume 9, Issue 4, pp 242–247 | Cite as

Abnormal expression of blood group-associated antigen (BGA) in colon of Hirschsprung's disease

  • Takao Fujimoto
  • Takeshi Miyano
Main Topic

Abstract

The entire segment of resected colon obtained from 15 patients with Hirschsprung's disease (HD) was examined with conventional mucin histochemistry (PASAB, HID-AB) to investigate the mucin composition and blood group-associated antigens (BGA) (sialosyl-Lea, Lea, Ley, Leb) for detecting mucosal cell differentiation. The BGA Leb were absent in normal distal colon and rectum but present in fetal colon. Immunoreactivity of Leb, which is a marker of undifferentiated crypt cells, was observed through out the crypts in aganglionic colon. Depletion of sulphated and neutral mucin was also a characteristic finding in the aganglionic mucosa. These findings suggest that the colonic mucosa of aganglionic bowel represents persistence of a fetal stage of development. These cellular abnormalities may be a factor in the pathogenesis of enterocolitis complicating HD.

Key words

Hirschsprung's disease Enterocolitis Blood group-associated antigens Mucin 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Takao Fujimoto
    • 1
  • Takeshi Miyano
    • 1
  1. 1.The Department of Paediatric Surgery Juntendo UniversitySchool of MedicineBankyo, Tokyo 113Japan

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