Amino Acids

, Volume 6, Issue 2, pp 205–211 | Cite as

Quantification of D-amino acids in human urine using GC-MS and HPLC

  • H. Brückner
  • S. Haasmann
  • A. Friedrich
Short Communication


The relative amounts of free D-amino acids (D-AA) in the urine of seven healthy volunteers (age 27 to 49 years) were determined using chiral phase (Chirasil-L-Val) capillary gas chromatography in conjunction with selected ion monitoring mass spectrometry. The absolute amounts of free D-AA were determined by pre-column derivatization of the amino acids witho-phthaldialdehyde andN-isobutyryl-L-cysteine followed by high-performance liquid chromatographic separation and fluorescence detection of the isoindol derivatives formed. The following most abundant D-AA were found (highest and lowest absolute and relative amounts): D-Ser (379.8 — 30.1µMol/L; 56.5 — 19.0%), D-Ala (53.8 — 7.6µMol/L; 19.6 — 5.7%), D-Thr (5.8 — 0.25µMol/L; 3.4 — 1.0%), D-Val (3.7 — 0µMol/L; 4.2 — 0%), and D-Phe (3.5 — 0.35µMol/L; 4.8 — 1.4%).


Amino acids Amino acid enantiomers Chiral analysis Chirasil-L-Val Gas chromatography Mass spectrometry Selected ion monitoring D-Serine D-Alanine 


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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • H. Brückner
    • 1
  • S. Haasmann
    • 1
  • A. Friedrich
    • 2
  1. 1.Institute of Food TechnologyUniversity of Hohenheim, Stuttgart, FederalStuttgartGermany
  2. 2.State Veterinary Laboratory StuttgartStuttgartGermany

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