Abstract
An attempt was made to find hypothetical structural changes in the cell membrane ofEscherichia coli under the influence of Ca++ cations with the aid of the uncharged fluorescent proble 4-dimethylaminochalcone (DMC). Effects of Tris buffer (0.01 M) at 0°C and of other agents, namely Mg++ cations and EDTA, also were tested for comparison. Treatment of theE. coli cells with Ca++ cations was shown to cause structural changes in the surface of the cell membrane which differed from the changes produced by treatment with Tris buffer at 0°C, Mg++ cations, and EDTA. DMC can be used with success as an indicator of structural changes in biomembranes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
G. E. Dobretsov, V. A. Petrov, A. I. Deev, et al., Biofizika,20, 1015 (1975).
G. E. Dobretsov, V. A. Petrov, A. I. Deev, et al., Biofizika,21, 459 (1976).
A. G. Sabel'nikov, T. F. Moiseeva, A. V. Avdeeva, et al., Biofizika,22, 640 (1977).
V. M. Sorokovoi, G. E. Dobretsov, V. A. Petrov, et al., Dokl. Akad. Nauk SSSR,206, 500 (1972).
L. Leive, Ann. N.Y. Acad. Sci.,235, 109 (1974).
M. Mandel and A. Higa, J. Molec. Biol.,53, 159 (1970).
A. G. Sabelnikov (A. G. Sabel'nikov), A. V. Avdeeva, and B. N. Ilyashenko [Il'yashenko], Molec. Gen. Genet.,138, 351 (1975).
L. Van Alphen, B. Lugtenberg, R. Van Boxtel, et al., Biochim. Biophys. Acta,466, 257 (1977).
A. Verkleij, L. Van Alphen, J. Bijvelt, et al., Biochim. Biophys. Acta,466, 269 (1977).
Rights and permissions
About this article
Cite this article
Sabel'nikov, A.G., Il'yashenko, B.N. Dimethylaminochalcone, an indicator of structural changes in theE. coli cell membrane produced by Ca++ cations and tris buffer. Bull Exp Biol Med 88, 747–750 (1979). https://doi.org/10.1007/BF00804784
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00804784