Some aspects of structural disturbances in the DNP complex when damaged by N-nitroso-N-methylurea
Biochemistry and Biophysics
Received:
- 17 Downloads
Abstract
The effect of solubilization of deoxyribonucleoproteins (DNP) in a medium of near-physiological ionic strength after treatment with the mutagen N-nitroso-N-methylurea (NMU) is highly dependent on the NMU concentration. To convert DNP into a soluble state, the critical number of groups in the DNA and protein must evidently be modified. On the basis of data obtained by the circular dichroism method and by viscosimetry it is concluded that after treatment with NMU the DNP complex becomes soluble in solvents with near-physiological ionic strength largely as a result of labilization and dissociation of the DNA-protein bonds.
Key Words
deoxyribonucleoproteins (DNP) DNA solubility of DNP single breaks in DNAPreview
Unable to display preview. Download preview PDF.
Literature Cited
- 1.B. S. Diskina, D. M. Spitkovskii, and V. S. Tongur, Biokhimiya, No. 3, 382 (1958).Google Scholar
- 2.V. I. Ivanov, in: Molecular Biology, Vol. 1 [in Russian], Moscow (1973), p. 105.Google Scholar
- 3.N. I. Ryabchenko, R. V. Golubeva, and B. P. Ivannik, Radiobiologiya, No. 2, 171 (1975).Google Scholar
- 4.P. I. Tseitlin, K. E. Kruglyakova, N. N. Zos, et al., Dokl. Akad. Nauk SSSR,222, 232 (1975).Google Scholar
- 5.É. V. Frisman, N. A. Sibileva, G. P. Pinaev, et al., Molekul. Biol., No. 3, 182 (1969).Google Scholar
- 6.J. Ramstein, C. Hèlène, and M. Leng, Europ. J. Biochem.,21, 125 (1971).Google Scholar
- 7.W. G. Verly, P. Crine, P. Bannon, et al., Biochim. Biophys. Acta349 204 (1974).Google Scholar
Copyright information
© Plenum Publishing Corporation 1976