Molecular Biology Reports

, Volume 13, Issue 4, pp 207–211 | Cite as

Evidence for a highly nuclease resistant RNA fragment in prosomes

  • Bistra Dineva
  • Wolfgang Tomek
  • Kurt Köhler
  • Hans-Peter Schmid
Article

Abstract

Prosomes, small cytoplasmic particles of mouse erythroblasts were found to contain low molecular weight RNA molecules in the range of 80 nucleotides. Nuclease digestion of prosomes suggests that prosomal proteins cover and protect almost the whole length of their RNA(s). Our results demonstrate clearly that RNA is an intrinsic component of prosomes.

Key words

prosomes cytoplasmic RNPs RNase resistance 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Schmid H-P, Akhayat O, Martins de Sa C, Puvion F, Köhler K & Scherrer K (1984) EMBO J. 3: 29–34Google Scholar
  2. 2.
    Martins de Sa C, Grossi de Sa M-F, Akhayat O, Broders F, Scherrer K, Horsch A & Schmid H-P (1986) J. Mol. Biol. 187: 479–493Google Scholar
  3. 3.
    Arrigo A-P, Darlix J-L, Khandjian EW, Simon M & Spahr P-F (1985) EMBO J. 4: 399–406Google Scholar
  4. 4.
    Arrigo AP, Simon M, Darlix J-L & Spahr P-FJ (1987) Mol. Evol. 25: 141–150Google Scholar
  5. 5.
    Kremp A, Schliephacke M, Kull U & Schmid HP (1986) Exp. Cell Res. 166: 553–557Google Scholar
  6. 6.
    Akhayat O, Grossi de Sa M-F & Infante AA (1987) Proc. Natl. Acad. Sci. USA 84: 1595–1599Google Scholar
  7. 7.
    Schult C & Kloetzel P-M (1985) Dev. Biol. 110: 65–74Google Scholar
  8. 8.
    Schmid H-P, Köhler K & Setyono B (1982) J. Cell Biol. 93: 893–898Google Scholar
  9. 9.
    Perry RP, LaTorre J, Kelley DE & Greenberg JR (1972) Biochim. Biophys Acta 262: 220–226Google Scholar
  10. 10.
    England E & Uhlenbeck O (1978) Nature 275: 560–561Google Scholar
  11. 11.
    Maniatis T, Fritsch EF & Sambrook J (1982) Molecular Cloning, a Laboratory Manual. CSHGoogle Scholar
  12. 12.
    O'Farrell PZ, Goodman H & O'Farrell PH (1977) Cell 12: 1133–1142Google Scholar
  13. 13.
    Kleinschmidt JA, Hügle B, Grund C & Franke WW (1983) Eur. J. Cell Biol. 32: 143–156Google Scholar
  14. 14.
    Arrigo A-P, Tanaka K, Goldberg AL, Welch WJ (1988) Nature 331: 192–194Google Scholar
  15. 15.
    Patton JR, Patterson RJ & Pederson T (1987) Mol. Cell Biol. 7: 4030–4037Google Scholar

Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Bistra Dineva
    • 1
  • Wolfgang Tomek
    • 1
  • Kurt Köhler
    • 1
  • Hans-Peter Schmid
    • 1
  1. 1.Biologisches InstitutUniversität StuttgartStuttgart 60FRG

Personalised recommendations