Journal of Bioenergetics and Biomembranes

, Volume 19, Issue 4, pp 341–350 | Cite as

Molecular genetics of the VDAC ion channel: Structural model and sequence analysis

  • Michael Forte
  • H. Robert Guy
  • Carmen A. Mannella
Mini-Review

Abstract

The voltage-dependent anion-selective channel of the outer mitochondrial membrane provides a unique system in which to study the molecular basis of voltage gating of ion flow. We have cloned and sequenced acDNA coding for this protein in yeast. From the derived amino acid sequence, we have generated a preliminary model for the secondary structure of the protein which suggests that the protein forms a “β-barrel” type structure. Comparison of the VDAC amino acid sequence with that of the bacterial porins has indicated that the two classes of molecules appear to be unrelated.

Kew Words

Yeast VDAC gene cloning secondary structure bacterial porins sequence homology 

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References

  1. Adrian, G., McCammon, M., Montgomery, D., and Douglas, M. (1986).Mol. Cell Biol. 6, 626.Google Scholar
  2. Aquila, T., Link, A., and Klingenberg, M. (1985).EMBO J. 4, 2369.Google Scholar
  3. Benz, R. (1985).CRC Crit. Rev. Biochem. 19, 145.Google Scholar
  4. Colombini, M. (1979).Nature (London)279, 643.Google Scholar
  5. Colombini, M. (1980).Ann N.Y. Acad. Sci. 341, 552.Google Scholar
  6. Colombini, M. (1983).J. Membr. Biol. 74, 115.Google Scholar
  7. Dayhoff, M. (1978).Atlas of Protein Sequence and Structure Vol. 5, Supplement 3, National Biomedical Research Foundation, Washington, D.C.Google Scholar
  8. Engel, A., Massalski, H., Schindler, D., Dorset, D., and Rosenbusch, J. (1985).Nature (London)317, 643.Google Scholar
  9. Forte, M., and Guy, R. (1986).Biophys. J. 49, 411a.Google Scholar
  10. Forte, M., Adelsberger-Mangan, D., and Colombini, M. (1987).J. Membr. Biol., in press.Google Scholar
  11. Freitag, H., Neupert, W., and Benz, R. (1982).Eur. J. Biochem. 123, 629.Google Scholar
  12. Freitag, H., Benz, R., and Neupert, W. (1983).Methods Enzymol. 258, 3427.Google Scholar
  13. Garnier, J., Osguthope, D., and Robson, B. (1978).J. Mol. Biol. 120, 97.Google Scholar
  14. Gasser, S. M., and Schatz, G. (1983).J. Biol. Chem. 254, 3427.Google Scholar
  15. Guy, R. (1983).Biophys. J. 41, 263a.Google Scholar
  16. Guy, R. (1984).Biophys. J. 45, 249.Google Scholar
  17. Guy, R. (1985).Biophys. J. 47, 61–70.Google Scholar
  18. Guy, R., and Seetharamulu, P. (1986).Proc. Natl. Acad. Sci. USA 83, 508.Google Scholar
  19. Linden, M., Gellerfors, P., and Nelson, D. (1982).Biochem. J. 208, 77.Google Scholar
  20. Lipman, D., and Pearson, W. (1985).Science 227, 1435.Google Scholar
  21. Mannella, C. A., and Auger, I. (1986).Biophys. J. 49, 272a.Google Scholar
  22. Mannella, C. A., Colombini, M., and Frank, J. (1983).Proc. Natl. Acad. Sci. USA 80, 2243.Google Scholar
  23. Mannella, C. A., Radermacher, M., and Frank, J. (1984).Proc. Annu. EMSA Meet. 42, 644.Google Scholar
  24. Mihara, K., and Sato, R. (1985).EMBO J. 4, 769.Google Scholar
  25. Mihara, K., Blobel, G., and Sato, R. (1982).Proc. Natl. Acad. Sci. USA 79, 7102.Google Scholar
  26. Roise, D., Horuath, S., Tomich, J., Richards, J., and Schatz, G. (1986).EMBO J. 5, 1327.Google Scholar
  27. Roos, N., Benz, R., and Brdiczka, D. (1982).Biochim. Biophys. Acta 682, 204.Google Scholar
  28. Rothstein, R. J. (1983).Methods in Enzymol. 101, 202.Google Scholar
  29. Schein, S., Colombini, M., and Finkelstein, A. (1976).J. Membr. Biol. 30, 99.Google Scholar
  30. Stevens, A., ten Heggeler, B., Muller, R., Kistler, J., and Rosenbusch, J. (1977).J. Cell Biol. 72, 292.Google Scholar
  31. Von Heijne, C. (1986).EMBO J. 5, 1335.Google Scholar

Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • Michael Forte
    • 1
  • H. Robert Guy
    • 2
  • Carmen A. Mannella
    • 3
  1. 1.Vollum Institute for Advanced Biomedical ResearchThe Oregon Health Sciences UniversityPortland
  2. 2.Laboratory of Mathematical BiologyNational Institutes of HealthBethesda
  3. 3.Wadsworth Center for Laboratories and ResearchNew York State Department of HealthAlbany

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