Journal of Wood Science

, Volume 48, Issue 6, pp 457–466 | Cite as

Immunohistochemical localization of enzymes related to lignin biosynthesis in the primary xylem of hybrid aspen

  • Yoshihiro KatayamaEmail author
  • Yoko Mashino
  • Nobuyuki Nishikubo
  • Kaori Yoshitomi
  • Ryo Funada
  • Shinya Kajita
Original Article


We have investigated the spatial regulation of the accumulation of enzymes involved in the biosynthesis of shikimate and lignin during differentiation of primary xylem from the apical meristem via procambium in hybrid aspen (Populus sieboldii x Populus grandidentata). Immuohistochemical staining revealed that, in the top part of shoots, lignification began in a single or just a few adjacent vessel elements and subsequently spread to neighboring cells. The spatial localization of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DAHPS), which is one of the key enzymes in the shikimate pathway, was tightly correlated with the cell-specific deposition of lignin in the primary xylem. We also found that the spatial localization of enzymes in the general phenylpropanoid pathway and in the lignin-specific pathway was closely associated with the cell-specific deposition of lignin and the accumulation of DAHPS. Our data suggest that enzymes that act in the shikimate, general phenylpropanoid, and lignin-specific pathways are initially produced and function coordinately in a single or a few adjacent elements at the start of primary xylem development.

Key words

Immunohistochemical localization Lignin deposition Phenylpropanoid pathway Primary xylem Shikimate pathway 


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Copyright information

© The Japan Wood Research Society 2002

Authors and Affiliations

  • Yoshihiro Katayama
    • 1
    Email author
  • Yoko Mashino
    • 1
  • Nobuyuki Nishikubo
    • 1
  • Kaori Yoshitomi
    • 1
  • Ryo Funada
    • 2
  • Shinya Kajita
    • 1
  1. 1.Department of Environment Symbiotic Production System, Graduate School of Bio-Applications and Systems EngineeringTokyo University of Agriculture and TechnologyTokyoJapan
  2. 2.Laboratory of Wood Biology, Graduate School of AgricultureHokkaido UniversitySapporoJapan

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