Journal of Bioenergetics and Biomembranes

, Volume 21, Issue 3, pp 387–401

Inhibition of the phosphate-stimulated cytochromec oxidase activity by thiophosphate

  • Stephen Manon
  • Nadine Camougrand
  • Martine Guerin
Research Articles

DOI: 10.1007/BF00762729

Cite this article as:
Manon, S., Camougrand, N. & Guerin, M. J Bioenerg Biomembr (1989) 21: 387. doi:10.1007/BF00762729

Abstract

Yeast and mammalian cytochromec oxidase activity is inhibited by thiophosphate. This inhibition was observed when using either whole mitochondria or the isolated or reconstituted enzyme. The kinetics of the reduction reaction enabled us to demonstrate that thiophosphate acted on th electrons transfer between hemesa anda3. With whole mitochondria, phosphate alone stimulated respiration. The inhibition induced by thiophosphate was suppressed by phosphate only in mitochondria, but not when the isolated enzyme was used. The possibility of a kinetic regulation is discussed.

Key Words

Cytochromec oxidase thiophosphate phosphate kinetic regulation 

Abbreviations

CCCP

p-carbonylcyanidem-chlorophenylhydrazone

TMPD

N,N,N′,N′-tetramethylp-phenylenediamine

SPi

thiophosphate

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Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • Stephen Manon
    • 1
    • 2
  • Nadine Camougrand
    • 1
    • 2
  • Martine Guerin
    • 1
    • 2
  1. 1.Institut de Biochimie Cellulaire et de Neurochimie du Centre National de la Recherche ScientifiqueBordeaux CedexFrance
  2. 2.Université de Bordeaux IIBordeauxFrance

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