Cytotechnology

, Volume 15, Issue 1–3, pp 11–16 | Cite as

Selecting and designing cell lines for improved physiological characteristics

  • J. R. Birch
  • R. C. Boraston
  • H. Metcalfe
  • M. E. Brown
  • C. R. Bebbington
  • R. P. Field
Article

Abstract

We have developed several approaches to create cell lines with improved characteristics in cell culture. In some cases it has been possible to isolate natural variants with useful properties. Cholesterol independent variants of the mouse NSO myeloma cell line were isolated by cloning in a selective medium. A glutamine independent variant of a hyridoma was isolated by continuous (chemostat) culture under glutamine limited conditions in the presence of glutamate. Choline independent cells were isolated from a choline limited chemostat. In an alternative approach to modifying cell behaviour, we have used recombinant DNA techniques to introduce the glutamine synthetase (GS) gene to a hybridoma. This resulted in glutamine independence and increased productivity.

Key words

Chemostat cholesterol choline glutamine glutamine synthetase hybridoma 
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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • J. R. Birch
    • 1
  • R. C. Boraston
    • 1
  • H. Metcalfe
    • 1
  • M. E. Brown
    • 1
  • C. R. Bebbington
    • 1
  • R. P. Field
    • 1
  1. 1.Celltech LimitedSloughUK

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