Cell Biology and Toxicology

, Volume 10, Issue 4, pp 247–254 | Cite as

Periodic fluctuations in proliferation of SV-40 transformed human skin fibroblast lines with prolonged lifespan

  • C. Wolfrom
  • N. Raynaud
  • J. Maigne
  • S. Papathanassiou
  • M. Conti
  • N. Kadhom
  • B. Hecquet
  • F. Levi
  • M. Gautier
  • J. Deschatrette
Article

Abstract

A human fibroblastic cell line transformed by the SV40-T antigen sequence and continuously cultured for 7 months displayed large periodic variations in cell proliferation. This contrasted with other characteristics of this cell line that remained constant: mosaic cell shape, absence of cell contact inhibition, and predominance of a hypodiploid population. Similar fluctuations in proliferative capacity were also found during the long-term growth of a transformed but nonimmortalized human fibroblastic line prior to senescence, and in the established hamster fibroblastic Nil cell line. This growth pattern suggests a recurrent stimulation of growth in these three transformed cell lines. The proliferation pattern from cultured transformed cells may thus be complex and requires further investigation. These variations presumably influence major cell functions. This observation has important implications for the analysis of data from such cell lines.

Keywords

proliferation periodicity transformed cultured cells fibroblasts 

Abbreviations

I-SF

immortalized human skin fibroblasts

T-SF

transformed human skin fibroblasts

FBS

fetal bovine serum

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • C. Wolfrom
    • 1
  • N. Raynaud
    • 1
  • J. Maigne
    • 1
  • S. Papathanassiou
    • 1
  • M. Conti
    • 1
  • N. Kadhom
    • 1
  • B. Hecquet
    • 2
  • F. Levi
    • 3
  • M. Gautier
    • 1
  • J. Deschatrette
    • 1
  1. 1.U56-U347 InsermHôpital BicêtreKremlin-Bicêtre CedexFrance
  2. 2.Laboratoire de Pharmacodynamie CliniqueCentre Oscar LambretLille CedexFrance
  3. 3.“Rythmes biologiques et Chronothérapeutique” (Université Paris XI)-Institut du Cancer et d'ImmunogénétiqueHôpital Paul BrousseVillejuif CedexFrance

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