Cell Biology and Toxicology

, Volume 9, Issue 3, pp 215–221 | Cite as

Effects of pyrethroid insecticides on gap junctional intercellular communications in Balb/c3T3 cells by dye-transfer assay

  • Chise Tateno
  • Seiichi Ito
  • Mina Tanaka
  • Akira Yoshitake
Reviews in Cell Toxicology


The effects of fenvalerate, esfenvalerate, permethrin, cypermethrin, deltamethrin, p-chlorophenylisovaleric acid (CPIA, major metabolite of fenvalerate) and DDT, a liver tumor promoter, on gap junctional intercellular communication (GJIC) were examined in Balb/c3T3 cells by dye-transfer assay. Separate groups of Balb/c3T3 cells were exposed to the chemicals for 1 day. On the following day, GJIC was measured by counting the number of dye-transferring cells per injection of Lucifer Yellow under a fluorescent microscope. Fenvalerate, esfenvalerate, permethrin, cypermethrin, deltamethrin and DDT inhibited GJIC at noncytotoxic concentrations, while CPIA did not inhibit GJIC even, at a cytotoxic concentration. It is concluded that the examined pyrethroid insecticides, but not a metabolite, have inhibitory effects on GJIC in Balb/c3T3 cells.

Key Words

Balb/c3T3 cypermethrin DDT deltamethrin dye-transfer assay esfenvalerate fenvalerate permethrin pyrethroids intercellular communication 





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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Chise Tateno
    • 1
  • Seiichi Ito
    • 1
  • Mina Tanaka
    • 1
  • Akira Yoshitake
    • 1
  1. 1.Environmental Health Science LaboratorySumitomo Chemical Co., LtdOsakaJapan

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