Fertilizer research

, Volume 40, Issue 2, pp 143–148

Assessing nitrogen mineralization from soil organic matter using anion exchange membranes

  • P. Qian
  • J. J. Schoenau


A simple method to assess differences in potential contribution of organic nitrogen mineralization to plant available N among soils may be useful in fertility research as well as routine soil testing. We deployed a method to assess mineralizable soil organic N using anion exchange membrane (AEM) burial. The method is based on a simple closed incubation system in which strips of AEM are buried directly in soil to adsorb NO3- released from organic matter. An index of mineralization was obtained using the amount of NO3- adsorbed on an AEM strip removed at the end of each incubation. The same incubation system but using 0.001M CaCl2 solution to extract NO3-N was used as the reference method. The mineralization indices obtained from both methods were compared with each other and with plant uptake. A total of 74 soils from across Saskatchewan were used in the study to provide a range of soil properties. Correlations between test values and N uptake by plants in two separate experiments showed the 2 week AEM incubation to be more closely correlated with plant N uptake (r2 = 0.86**** and 0.57****, respectively) than the reference method (r2 = 0.60**** and 0.48****, respectively).

With this method, we were able to determine the influence of different tillage systems and landscape positions on mineralizable N. The results showed that the NO3- released from soil organic matter and accumulated on the AEM reflected the expected effect of three different tillage systems and two landscape positions on mineralizable N. Cropping systems with continuous alfalfa (Medicago sativa) showed higher N release from soil organic matter than a canola (Brassica napus)-lentil (Lens culinaris)-barley (Hordeum vulgare) rotation did. Higher N mineralization was found in the lower slope positions of the landscape where organic matter contents are highest. Direct burial of AEM appears to be a simple and effective method of including a measure of N mineralization in a soil test.

Key words

anion exchange membrane incubation N mineralization organic matter slope tillage 


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  1. Bremner JM (1965) Total Nitrogen.In: Black CA et al. (ed) Methods of Soil Analysis, Part 2. Agronomy 9:1149-1176. Am Soc Agron., Inc., Madison, WIGoogle Scholar
  2. Campbell CA, LaFond GP, Leyshon AJ, Zentner RP and Janzen HH (1991) Effect of cropping practices on the initial potential rate of N mineralization in a thin Black Chernozem. Can J Soil Sci 71: 43–53Google Scholar
  3. Carson PL (1975) Recommended nitrate-nitrogen tests.In: Dahnke WC (ed.) Recommended Chemical Soil Test Procedures for the North-Central Region. North Dakota Agric Exp. Stn Bull 499, pp 13–15. North Dakota State Univ, FargoGoogle Scholar
  4. Carter JN, Jensen ME and Bosma SM (1974) Determining nitrogen fertilizer needs for sugar beets from residual nitrate and mineralizable nitrogen. Agron J 66: 319–323Google Scholar
  5. Carter JN, Westerman DT and Jensen ME (1976) Sugar yield and quality as affected by nitrogen level. Agron J 68: 49–55Google Scholar
  6. Cook FD, Warder FG and Doughty JL (1957) Relationship of nitrate accumulation to yield response of wheat in some Saskatchewan soils. Can J Soil Sci 37: 84–88Google Scholar
  7. Herron GM, Dreier AF, Flowerday AD, Colville WL and Olsen RA (1971) Residual mineral N accumulation in soil and its utilization by irrigated corn (Zea mays L.). Agron J 63: 322–327Google Scholar
  8. Keeney DR and Nelson DW (1982) Nitrogen-inorganic form.In: Black CA et al. (ed) Methods of Soil Analysis, Part 2. Agronomy 9: 643-698. Am Soc Agron, Inc, Madison, WIGoogle Scholar
  9. Maynard DG, Stewart JWB and Bettany JR (1983) Sulfur and nitrogen mineralization in soils compared using two incubation system techniques. Soil Biol Biochem 15: 251–256Google Scholar
  10. Malo DD, Worcester BK, Cassel DK and Matzdorf KD (1974) Soil-landscape relationships in a closed drainage system. Soil Sci Soc Am Proc 38: 813–818Google Scholar
  11. Nyborg M and Hoyt PB (1978) Effects of soil acidity and liming on mineralization of soil nitrogen. Can J Soil Sci 58: 331–338Google Scholar
  12. Qian P, Schoenau JJ and Huang WZ (1992) Use of ion exchange membranes in routine soil testing. Commun Soil Sci Plant Anal 23: 1791–1804Google Scholar
  13. Rixon AJ (1969) The influence of annual and perennial irrigated pastures on soil fertility as shown by the yield and quality of a subsequent wheat crop. Aust J Agric Res 20: 243–255Google Scholar
  14. Roberts TL (1985) Sulfur and its relationship to carbon, nitrogen and phosphorus in a climo-oposequence of Saskatchewan soils. Ph D thesis. University of Saskatchewan, CanadaGoogle Scholar
  15. Sagger S, Hedley MJ and White RE (1990) A simplified resin membrane technique for extracting phosphorus from soils. Fert Res 24: 173–180Google Scholar
  16. Schoenau JJ, Qian P and Huang WZ (1993) Assessing sulphur availability in soil using ion exchange membranes. Sulphur Agric 17: 13–17Google Scholar
  17. Smith JA (1966) An evaluation of nitrogen soil test method for Ontario soils. Can J Soil Sci 46: 185–194Google Scholar
  18. Soper RJ, Racz GL and Fehr PL (1971) Nitrate nitrogen in the soil as a means of predicting the fertilizer nitrogen requirements of barley. Can J Soil Sci 51: 45–49Google Scholar
  19. Stanford G, Ayers AS and Doi M (1965) Mineralizable soil nitrogen in relation to fertilizer needs of sugarcane in Hawaii, Soil Sci 99: 132–137Google Scholar
  20. Stanford G and Smith SJ (1972) Nitrogen mineralization potentials of soils. Soil Sci Soc Am Proc 36: 465–472Google Scholar
  21. Stanford G, Carter JN, Westerman DT and Meisinger JJ (1977) Residual nitrate and mineralizable soil nitrogen in relation to nitrogen uptake by irrigated sugar beets. Agron J 69: 303–308Google Scholar
  22. Thomas R, Sheard RW and Moyer JP (1967) Comparison of conventional and automated procedures for nitrogen, phosphorus and potassium analysis of plant material using a single digest. Agron J 99: 240–243Google Scholar
  23. van Raij B, Quaggio JA and da Silva NM (1986) Extraction of phosphorus, potassium, calcium and magnesium from soils by using an ion-exchange resin procedure. Commun Soil Sci Plant Anal 17: 547–566Google Scholar
  24. Vlassak K (1970) Total soil nitrogen and nitrogen mineralization. Plant Soil 32: 27–32Google Scholar

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • P. Qian
    • 1
  • J. J. Schoenau
    • 1
  1. 1.Department of Soil ScienceUniversity of SaskatchewanSaskatoonCanada

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