Effects of peptone on hybridoma growth and monoclonal antibody formation
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Abstract
Hybridoma WuT3 secreting a monoclonal antibody against T lymphocytes was grown in RPMI 1640 medium supplemented with 1% human serum. The effect of the concentration of peptone, as an additive, was investigated on cell growth, monoclonal antibody formation, and cell metabolism over 0–10 g l−1 range. It was found that 1–5 g l−1 peptone can significantly promote the growth of cells and increase the formation of monoclonal antibody, especially at 3–5 g l−1, when both the accumulating level and secretion rate of monoclonal antibody are higher than that at other peptone concentrations. Based on glucose, lactate and ammonia analysis data, the efficiency of glycolysis was assessed and the utilization of amino acids was more efficient at 3–5 g l−1 peptone. The cell growth and monoclonal antibody formation were inhibited at higher peptone concentrations, e.g. 10 g l−1.
Key words
Hybridoma peptone monoclonal antibody cell culturePreview
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References
- Bols NC, Scharer JM, Phillips HA and Moo-Young M (1988) Medium for hybridoma growth and monoclonal antibody production. Biotechnol. Adv. 6: 169–182.Google Scholar
- Duval D, Demangel C, Munier-Jolain K, Miossec S and Geahel I (1991) Factors controlling cell proliferation and antibody production in mouse hybridoma cells: 1. Influence of the amino acid supply. Biotechnol. Bioeng. 38: 561–570.Google Scholar
- Graf H and Schugerl K (1991) Some aspects of hybridoma cell cultivation. Appl. Microbiol. Biotechnol. 36: 165–175.Google Scholar
- Keay L (1975) Autoclavable low cost serum-free cell culture media: The growth of L cells and BHK cells on peptones. Biotechnol. Bioeng. 17: 745–764.Google Scholar
- Keay L (1976) Autoclavable low cost serum-free cell culture media: The growth of established cell lines and production of viruses. Biotechnol. Bioeng. 18: 363–382.Google Scholar
- Keay L (1977) The growth of L cells and Vero cells on an autoclavable MEM-peptone medium. Biotechnol. Bioeng. 19: 399–411.Google Scholar
- Liu F, Zhang Y, Zhang L, Chen Z and Yu J (1992) Effects of nutrients and metabolites on the growth of hybridoma cells. J. East China Univ. of Chem. Technol. 18: 286–291.Google Scholar
- Low K and Harbour S (1985) Growth kinetics of hybridoma cells: (1) the effects of varying foetal calf serum levels. Dev. Biol. Stand. 60: 17–24.Google Scholar
- Mather JP and Tsao MC (1992) Culture medium for improved yields of heterologous proteins from transgenic animal cells. US Pat. 5122569.Google Scholar
- McQueen A and Bailey JE (1990) Effect of ammonium ion and extracellular pH on hybridoma cell metabolism and antibody production. Biotechnol. Bioeng. 35: 1067–1077.Google Scholar
- Shacter E (1987) Serum-free medium for growth factor-dependent and-independent plasmacytomas and hybridomas. J. Immunol. Methods 99: 259–270.Google Scholar
- Thomas JN (1990) Mammalian cell physiology. In: Lubiniecki AS (ed), Large-scale mammalian cell culture technology (pp. 93–145). Marcel Dekker, New York.Google Scholar
- Zhang G and Rong B (1987) The applications of monoclonal antibodies in medicine. Shanghai Press of Science and Technology, Shanghai.Google Scholar