Cytotechnology

, Volume 11, Issue 3, pp 169–174 | Cite as

A serum-free medium for hybridoma cell culture

  • Zhihong Chen
  • Yongzhong Ke
  • Yinliang Chen
Original Research Papers
Received:
Accepted:

Abstract

The effects of several different substances, including insulin, transferrin, ethanolamine, selenite and butyrate on the growth of murine hybridoma 2F7 cells, which secrete monoclonal antibody against small cell lung cancer, were investigated, and a serum-free medium SFMI was formulated. The effects of taurine, spermidine, progesterone and adenine on the cell growth were tested further on the basis of the medium SFMI, and a modified serum-free medium SFM II was established. On the basis of medium SFM II, the substitution tests of ferric citrate for transferrin were carried out, and it was found that transferrin could be replaced. The experiments suggested that the formulated serum-free medium was suitable for 2F7 cell growth and monoclonal antibody secretion, and thus facilitated subsequent purification of monoclonal antibody.

Key words

cell culture hybridoma monoclonal antibody serum-free medium 

Abbreviations

BSA

bovine serum albumin

CS

calf serum

DMEM

Dulbecco's modified Eagle's medium

ELISA

enzyme-linked immunosorbant assay

McAb

monoclonal antibody

PEG

polyethylene glycol

SFM

serum-free medium

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Zhihong Chen
    • 1
  • Yongzhong Ke
    • 1
  • Yinliang Chen
    • 1
  1. 1.Research Institute of Biochemical EngineeringEast China University of Chemical TechnologyShanghaiChina

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