Cytotechnology

, Volume 19, Issue 1, pp 27–36 | Cite as

CHO cell growth and recombinant interferon-γ production: Effects of BSA, Pluronic and lipids

  • Paula M. L. Castro
  • Andrew P. Ison
  • Paul M. Hayter
  • Alan T. Bull
Article

Abstract

The role of bovine serum albumin in mammalian cell cultures and the possibility of its substitution by other components in a serum-free medium has been investigated. In this study, BSA was shown to be important for growth and product formation in CHO cells expressing recombinant human interferon-γ. There were indications that its stimulating growth effect was dependent on the source of BSA used and probably was related to the purification procedure used for the production of the desired albumin fraction. Cell growth did not occur in the absence of BSA but at low concentration (1 mg ml−1) it was stimulated by the addition of a combination of a commercial lipid mixture plus Pluronic F68. However, under the latter conditions IFN-γ production was adversely effected. The importance of individual lipid components was investigated using a statistical approach based on a Plackett-Burman design. Linoleic acid was identified as a positive variable for cell growth while cholesterol was identified as a negative variable for both cell growth and IFN-γ production. When a combination of linoleic acid plus Pluronic F68 was included in the formulation of low BSA medium, cell growth was similar to that at high BSA concentration (5 mg ml−1) but the IFN-γ concentration was significantly reduced (ca. 45%).

Key words

BSA CHO cells interferon-γ linoleic acid lipids Pluronic F68 

Abbreviations

IFN-γ

interferon-γ

CHO

Chinese Hamster Ovary cells

BSA

bovine serum albumin

FAF-BSA

fatty acid-free bovine serum albumin

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Paula M. L. Castro
    • 1
    • 2
  • Andrew P. Ison
    • 2
  • Paul M. Hayter
    • 1
  • Alan T. Bull
  1. 1.Research School of Biosciences, Biological LaboratoryUniversity of KentCanterburyUK
  2. 2.The Advanced Centre for Biochemical EngineeringUniversity College LondonLondonUK

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