Glycoconjugate Journal

, Volume 10, Issue 2, pp 142–151 | Cite as

Synthesis of polymeric neoglycoconjugates based onN-substituted polyacrylamides

  • Nicolai V. Bovin
  • Elena Yu. Korchagina
  • Tatyana V. Zemlyanukhina
  • Nargiz E. Byramova
  • Oksana E. Galanina
  • Alexandr E. Zemlyakov
  • Alexandr E. Ivanov
  • Vitaly P. Zubov
  • Larisa V. Mochalova


Several types of polymeric glycoconjugates,N-substituted polyacrylamides, have been synthesized by the reaction of activated polymers with ω-aminoalkylglycosides: (i) (carbohydrate-spacer) n -polyacrylamide, ‘pseudopolysaccharides’; (ii) (carbohydrate-spacer) n -phosphatidylethanolamine m -polyacrylamide, neoglycolipids, derivatives of phosphatidylethanolamine; (iii) (carbohydrate-spacer) n -biotin m -polyacrylamide, biotinylated probes; (iv) (carbohydrate-spacer) n -polyacrylamide-(macroporous glass), affinity sorbents based on macroporous glass, covalently coated with polyacrylamide. An almost quantitative yield in the conjugation reaction makes it possible to insert in the conjugate a predetermined quantity of the ligand(s).

Pseudopolysaccharides proved to be a suitable form of antigen for activation of polystyrene and poly(vinyl chloride) plates (ELISA) and nitrocellulose membranes (dot blot), being advantageous over traditional neoglycoproteins. Polyvalent glycolipids insert well in biological membranes: their physical properties, particularly solubility, can be changed in a desired direction. Biotinylated derivatives were used as probes for detection and analysis of lectins.


Polymer Chloride Polyacrylamide Polystyrene Vinyl 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



spacer arm








macroporous glass 200 Å


enzyme-linked immunosorbent assay


high-performance liquid chromatography




bovine serum albumin




thin-layer chromatography






refractive index


phosphate buffered saline (0.14m NaCl, 0.01m sodium phosphate, pH 7.3)


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Copyright information

© Chapman & Hall 1993

Authors and Affiliations

  • Nicolai V. Bovin
    • 1
  • Elena Yu. Korchagina
    • 1
  • Tatyana V. Zemlyanukhina
    • 1
  • Nargiz E. Byramova
    • 1
  • Oksana E. Galanina
    • 1
  • Alexandr E. Zemlyakov
    • 1
  • Alexandr E. Ivanov
    • 1
  • Vitaly P. Zubov
    • 1
  • Larisa V. Mochalova
    • 1
  1. 1.Shemyakin Institute of Bioorganic ChemistryAcademy of Sciences, MoscowMoscowRussian Federation

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