Virchows Archiv A

, Volume 412, Issue 3, pp 273–279 | Cite as

Osteosarcoma with a clear-cell component

  • Ctibor Povýšil
  • Zdeněk Matějovský
  • Helena Zídková


The reports concerns the light microscopical and ultrastructural findings obtained in three conventional osteosarcomas with an unusually high admixture of clear cells, whose presence appeared to be responsible for the marked change in the histological pattern of these tumours. In the tumours with a prevailing fibroblastic component the clear cells were either irregularly scattered throughout the tumour in the form of small groups, or they formed large groups sharply demarcated against the fusicellular areas of the tumours. In two cases it was shown that their cytoplasm contained exaggerated glycogen deposits accompanied by the formation of glycogen-containing phagolysosomes and occasional empty vacuoles. In the third case the clear cells showed vacuolar degeneration with numerous single-membrane-bound, empty vacuoles. In contrast to the clear-cell chondrosarcoma we did not find S-100 protein in clear cells of our osteosarcomas. Such findings could be particularly significant in the differential diagnosis of bone tumours.

Key words

Osteosarcoma Clear cell Histopathology Electron microscopy 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Angervall L, Kindblom LG (1980) Clear-cell chondrosarcoma: A light- and electron-microscopic and histochemical study of two cases. Virchows Arch A (Pathol Anat) 389:27–41Google Scholar
  2. Bjornsson J, Beabout JW, Unni KK, Sim FH, Dahlin DC (1984) Clear-cell chondrosarcoma of bone. Observations in 47 cases. Am J Surg Pathol 8:223–230Google Scholar
  3. Dahlin DC, Unni KK (1986) Bone tumours. Fourth edition, Charles C Thomas, Sprinfield-IllinoisGoogle Scholar
  4. Erlandson RA, Tandler B, Lieberman PH, Higinbothan NL (1968) Ultrastructure of human chordoma. Cancer Res 28:2115–2125Google Scholar
  5. Faraggiana T, Sender Y, Glicksman P (1981) Light- and electron-microscopic study of clear-cell chondrosarcoma. Am J Clin Pathol 75:117–121Google Scholar
  6. Huvos AG (1979) Bone tumors. Diagnosis, treatment and prognosis. WB Saunders Company, Philadelphia, London, TorontoGoogle Scholar
  7. Meister P, Konrad EA, Wallmüller-Strycker A (1980) Metastasiertes Osteosarkom mit undifferenzierten karzinomähnlichen Anteilen. Arch Orthop Traumat Surg 96:75–78Google Scholar
  8. Mirra JM (1980) Bone tumors, diagnosis and treatment. JB Lippincott Company, Philadelphia, TorontoGoogle Scholar
  9. Povýšil C, Matějovský Z (1977) Ultrastructure of Ewing's tumour. Virchows Arch A (Pathol Anat) 374:303–316Google Scholar
  10. Povýšil C, Matějovský Z (1985) A comparative ultrastructural study of chondrosarcoma, chordoid sarcoma, chordoma and chordoma periphericum. Pathol Res Pract 179:546–559Google Scholar
  11. Povýšil C (1986) Histopathology and ultrastructure of tumours and tumour-like lesions of bone. Acta Univ Carol Med, Monographia 116, PragueGoogle Scholar
  12. Roessner A (1984) Zur Zyto- und Histogenese der malignen und semimalignen Knochentumoren. Veröffentl a d Pathol, Heft 122, Gustav Fisher, Stuttgart, New YorkGoogle Scholar
  13. Salzer-Kuntschik M (1981) Clear-cell chondrosarcoma. J Cancer Res Clin Oncol 101:171–176Google Scholar
  14. Schajowicz F (1981) Tumors and tumor-like lesions of bone and joints. Springer, Berlin, Heidelberg, New YorkGoogle Scholar
  15. Schulz A (1980) Ultrastrukturpathologie der Knochentumoren. Veröffentl a d Pathol, Heft 115, Gustav Fisher, Stuttgart, New YorkGoogle Scholar
  16. Scranton PE, De Cicco FA, Totten RS, Yunis EJ (1975) Prognostic factors in osteosarcoma. Cancer 36:2179–2191Google Scholar
  17. Spjut HJ, Dorfman HD, Fechner RE, Ackerman LV (1971) Tumors of bone and cartilage. Atlas of tumor pathology, 2nd series, Fasc 5, AFIP, Washington DCGoogle Scholar
  18. Unni KK, Dahlin DC, Beabout JW, Sim FH (1976) Chondrosarcoma: Clear-cell variant: a report of sixteen cases. J Bone Joint Surg Am 58:676–684Google Scholar
  19. Weiss APC, Dorfman HD (1986) S-100 protein in human cartilage lesions. J Bone Joint Surg Am 68:521–526Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Ctibor Povýšil
    • 1
  • Zdeněk Matějovský
    • 2
  • Helena Zídková
    • 3
  1. 1.2nd Institute of Pathology, Faculty of General MedicineCharles UniversityPraha 2Czechoslovakia
  2. 2.Orthopedic ClinicInstitute for Postgraduate StudiesPragueCzechoslovakia
  3. 3.Radiodiagnostic ClinicInstitute for Postgraduate StudiesPragueCzechoslovakia

Personalised recommendations