, Volume 5, Supplement 1, pp 132–133 | Cite as

A growth factor different from IL-6 promotes the growth of Epstein-Barr virus-transformed cells at low cell density

  • Claudia Negri
  • Antonella Cerino
  • Roberto Chiesa e
  • Giulia C. B. Astaldi Ricotti
Selected Paper


Interleukin-6 (IL-6) was recently described to support the growth of Epstein-Barr (EBV) transformed lymphocytes. However no effect was reported on cloning of EBV transformed lymphocytes under limiting dilution conditions. In this paper we demonstrate that the Supernatant of Human Endothelial Cells (HECS) contains a factor(s) different from IL-6 which induces high cloning efficiency of EBV transformed cell lines, cultured under limiting dilution conditions. Furthermore HECS is superior to IL-6 in all the passages of EBV lymphocytes transformation.


Growth Factor Endothelial Cell Cell Density Transformed Cell Human Endothelial Cell 
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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Claudia Negri
    • 1
  • Antonella Cerino
    • 1
  • Roberto Chiesa e
    • 1
  • Giulia C. B. Astaldi Ricotti
    • 1
  1. 1.Istitute of Biochem. and Evol. Genetic of C.N.R.PaviaItaly

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