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Cytotechnology

, Volume 5, Supplement 1, pp 132–133 | Cite as

A growth factor different from IL-6 promotes the growth of Epstein-Barr virus-transformed cells at low cell density

  • Claudia Negri
  • Antonella Cerino
  • Roberto Chiesa e
  • Giulia C. B. Astaldi Ricotti
Selected Paper
  • 17 Downloads

Abstract

Interleukin-6 (IL-6) was recently described to support the growth of Epstein-Barr (EBV) transformed lymphocytes. However no effect was reported on cloning of EBV transformed lymphocytes under limiting dilution conditions. In this paper we demonstrate that the Supernatant of Human Endothelial Cells (HECS) contains a factor(s) different from IL-6 which induces high cloning efficiency of EBV transformed cell lines, cultured under limiting dilution conditions. Furthermore HECS is superior to IL-6 in all the passages of EBV lymphocytes transformation.

Keywords

Growth Factor Endothelial Cell Cell Density Transformed Cell Human Endothelial Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Claudia Negri
    • 1
  • Antonella Cerino
    • 1
  • Roberto Chiesa e
    • 1
  • Giulia C. B. Astaldi Ricotti
    • 1
  1. 1.Istitute of Biochem. and Evol. Genetic of C.N.R.PaviaItaly

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