Glycoconjugate Journal

, Volume 13, Issue 2, pp 177–186 | Cite as

Isolation of three novel cholinergic neuron-specific gangliosides from bovine brain and theirin vitro syntheses

  • Fumitoshi Irie
  • Sadamu Kurono
  • Yu-Teh Li
  • Yousuke Seyama
  • Yoshio Hirabayashi
Papers
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Revised:

Abstract

In the present study, three extremely minor but novel Chol-1 antigens, termed X1, X2, and X3 have been isolated from bovine brain gangliosides. Based on the results of sialidase degradation, TLC-immunostaining with anti-Chol-1 antibody and fast atom bombardment mass spectrometry, their chemical structures were identified as:
$$\begin{gathered} III^6 NeuAc--GgOse4Cer (X1:GM1\alpha ) \hfill \\ III^6 NeuAc,II^3 NeuAc--GgOse4Cer (X2:GT1a\alpha ) \hfill \\ III^6 NeuAc,II^3 NeuAc--NeuGc--GgOse4Cer (X3:GT1b\alpha ) \hfill \\ \end{gathered} $$
The yields of GM1α, GD1aα, and GT1bα, were approximately 150, 20, and 10 µg, respectively, from 10 g of the bovine brain ganglioside mixture. In conjunction with our previous observations, all gangliosides with anti-Chol-1 reactivity were found to contain a common sialyl α2–6N-acetylgalactosamine residue, indicating that this unique sialyl linkage is the specific antigenic determinant. We subsequently examined the biosyntheses of the three novel Chol-1 gangliosides using rat liver Golgi fraction as an enzyme source. The results showed that GM1α, GD1aα, and GT1bα were synthesized from asialo-GM1, GM1a, and GD1b, respectively, by the action of a GalNAc α2-6sialyltransferase.

Keywords

ganglioside cholinergic neuron ganglioside biosynthesis sialyltransferase 
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References

  1. 1.
    Jones RT, Walker JH, Richardson PJ, Fox GQ, Whittaker VP (1981)Cell Tissue Res 218: 355–73.Google Scholar
  2. 2.
    Walker JH, Jones TJ, Obrocki J, Richardson GP, Stadlar H (1982)Cell Tissue Res 223: 101–16.Google Scholar
  3. 3.
    Richardson PJ, Walker KH, Jones RT, Whittaker VP (1982)J Neurochem 38: 1605–14.Google Scholar
  4. 4.
    Ferretti P, Borroni E (1986)J Neurochem 46: 1888–94.Google Scholar
  5. 5.
    Obrokki J, Borroni E (1988)Exp Brain Res 72: 71–82.Google Scholar
  6. 6.
    Giuliani A, Calappi E, Borroni E, Whittaker VP, Sonnino S, Tettamanti G (1990)Arch Biochem Biophys 280: 211–16.Google Scholar
  7. 7.
    Hirabayashi Y, Nakao T, Matsumoto M, Obata K, Ando S (1988)J Chromatogr 445: 377–84.Google Scholar
  8. 8.
    Hirabayashi Y, Hyogo A, Nakao T, Tsuchiya K, Suzuki Y, Matsumoto M, Kon K, Ando S (1990)J Biol Chem 265: 8144–51.Google Scholar
  9. 9.
    Hidari KI-PJ, Irie F, Suzuki M, Kon K, Ando S, Hirabayashi Y (1993)Biochem J 296: 259–63.Google Scholar
  10. 10.
    Nakao T, Kon K, Ando S, Miyatake T, Yuki N, Li Y-T, Furuya S, Hirabayashi Y (1993)J Biol Chem 268: 21028–34.Google Scholar
  11. 11.
    Ando S, Hirabayashi Y, Kon K, Inagaki F, Tate S, Whittaker VP (1992)J Biochem (Tokyo) 111: 287–90.Google Scholar
  12. 12.
    Hirabayashi Y, Nakao T, Irie F, Whittaker VP, Kon K, Ando, S (1992)J Biol Chem 267: 12973–78.Google Scholar
  13. 13.
    Li Y-T, Nakagawa H, Ross SA, Hansson GC, Li S-C (1990)J Biol Chem 265: 21629–33.Google Scholar
  14. 14.
    Chou M-Y, Li S-C, Kiso M, Hasegawa A, Li Y-T (1994)J Biol Chem 269: 18821–26.Google Scholar
  15. 15.
    Hirabayashi Y, Suzuki T, Suzuki Y, Taki T, Matsumoto M, Higashi H, Kato S (1983)J Biochem (Tokyo) 94: 327–30.Google Scholar
  16. 16.
    Higashi H, Fukui Y, Ueda S, Kato S, Hirabayashi Y, Matsumoto M, Naiki, M (1984)J Biochem (Tokyo) 95: 1517–20.Google Scholar
  17. 17.
    Higashi H, Ikuta K, Ueda S, Kato S, Hirabayashi Y, Matsumoto M, Naiki M (1984)J Biochem (Tokyo) 95: 785–94.Google Scholar
  18. 18.
    Ohashi Y, Kurono S (1994) InBiological Mass Spectrometry: Present and Future (Matsuo T, Caprioli RM, Gross ML, Seyama Y, eds) pp. 647–653, Chichester: John Wiley & Sons Ltd.Google Scholar
  19. 19.
    Sandberg P-O, Marzella L, Glumann H (1980)Exp Cell Res 130: 393–400.Google Scholar
  20. 20.
    Hidari KI-PJ, Sanai Y, Kawashima I, Tai T, Inagaki F, Nagai Y (1994)Eur J Biochem 221: 603–9.Google Scholar
  21. 21.
    Irie F, Hidari KI-PJ, Tai T, Li Y-T, Seyama Y, Hirabayashi Y (1994)FEBS Lett 351: 291–94.Google Scholar
  22. 22.
    Nakao T, Kon K, Ando S, Hirabayashi Y (1991)Biochim Biophys Acta 1086: 305–9.Google Scholar
  23. 23.
    Schachter H, Roseman S (1980) InThe Biochemistry of Glycoproteins and Proteoglycans (Lennarz WJ, ed.) pp. 85–160, New York: Plenum Press.Google Scholar
  24. 24.
    Pohlentz G, Klein D, Schmitz D, Schwarzmann G, Peter-Katalinic J, Sandhoff K (1988)Biol Chem Hoppe-Seyler 369: 55–63.Google Scholar
  25. 25.
    Pohlentz G, Klein D, Schwarzmann G, Schmitz D, Sandhoff K (1988)Proc Natl Acad Sci 85: 7044–48.Google Scholar
  26. 26.
    Iber H, Kaufmann R, Pohlentz G, Schwarzmann G, Sandhoff K (1989)FEBS Lett 248: 18–22.Google Scholar
  27. 27.
    Iber H, Sandhoff K (1989)FEBS Lett 254: 124–28.Google Scholar
  28. 28.
    Kusunoki S, Chiba A, Hirabayashi Y, Irie F, Kotani M, Kawashima I, Tai T, Nagai Y (1993)Brain Res 623: 83–88.Google Scholar
  29. 29.
    Irie F, Hashikawa T, Seyama Y, Hirabayashi Y (1993)Glycoconjugate J 10: 292–93.Google Scholar
  30. 30.
    Irie F, Hashikawa T, Tai T, Seyama Y, Hirabayashi Y (1994)Brain Res 665: 161–66.Google Scholar
  31. 31.
    Fukunaga K, Miyamoto E, Soderling TR (1990)J Neurochem 54: 102–9.Google Scholar
  32. 32.
    Higashi H, Yamagata T (1992)J Biol Chem 267: 9839–43.Google Scholar
  33. 33.
    Higashi H, Omori A, Yamagata T (1992)J Biol Chem 267: 9831–38.Google Scholar
  34. 34.
    Bremer EG, Hakomori S, Bowen-Pope DF, Raines E, Ross R (1984)J Biol Chem 259: 6818–25.Google Scholar
  35. 35.
    Bremer EG, Schlessinger J, Hakomori S (1986)J Biol Chem 261: 2434–40.Google Scholar
  36. 36.
    Hara-Yokoyama M, Hirabayashi Y, Irie F, Syuto B, Moriishi K, Sugiya H, Furuyama S (1995)J Biol Chem 270: 8115–21.Google Scholar
  37. 37.
    Svennerholm L (1964)Lipids 5: 145–62.Google Scholar

Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Fumitoshi Irie
    • 1
    • 2
  • Sadamu Kurono
    • 1
  • Yu-Teh Li
    • 3
  • Yousuke Seyama
    • 2
  • Yoshio Hirabayashi
    • 1
  1. 1.Laboratory for Cellular GlycoBiology, Frontier Research ProgramThe Institute of Physical and Chemical Research (RIKEN)SaitamaJapan
  2. 2.Department of Physiological Chemistry and Nutrition, Faculty of MedicineThe University of TokyoTokyoJapan
  3. 3.Department of BiochemistryTulane University School of MedicineNew OrleansUSA

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