Glycoconjugate Journal

, Volume 13, Issue 4, pp 687–692

One-pot enzymatic synthesis of the Galα1→3Galβ1→4GlcNAc sequence within situ UDP-Gal regeneration

  • Cornelis H. Hokke
  • Astrid Zervosen
  • Lothar Elling
  • David H. Joziasse
  • Dirk H. van den Eijnden
Glycoconjugate Journal

DOI: 10.1007/BF00731458

Cite this article as:
Hokke, C.H., Zervosen, A., Elling, L. et al. Glycoconjugate J (1996) 13: 687. doi:10.1007/BF00731458

Abstract

The trisaccharide Galα1→3Galβ1→4GlcNAcβ1→O-(CH2)8COOCH3 was enzymatically synthesized, within situ UDP-Gal regeneration. By combination in one pot of only four enzymes, namely, sucrose synthase, UDP-Glc 4′-epimerase, UDP-Gal:GlcNAc β4-galactosyltransferase and UDP-Gal:Galβ1→4GlcNAc α3-galactosyltransferase, Galα1→3Galβ1→4GlcNAcβ1→O-(CH2)8COOCH3 was formed in a 2.2 µmol ml−1 yield starting from the acceptor GlcNAcβ1→O-(CH2)8COOCH3. This is an efficient and convenient method for the synthesis of the Galα1→3Galβ1→4GlcNAc epitope which plays an important role in various biological and immunological processes.

Keywords

α3-galactosyltransferase sucrose synthase enzymatic synthesis UDP-Gal 

Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Cornelis H. Hokke
    • 1
  • Astrid Zervosen
    • 2
  • Lothar Elling
    • 2
  • David H. Joziasse
    • 1
  • Dirk H. van den Eijnden
    • 1
  1. 1.Department of Medical ChemistryVrije UniversiteitAmsterdamThe Netherlands
  2. 2.Institute for Enzyme TechnologyHeinrich-Heine Universität Düsseldorf, Research Center JülichJülichGermany

Personalised recommendations