Glycoconjugate Journal

, Volume 11, Issue 6, pp 593–599

Expression of Lewis histo-blood group glycolipids in the plasma of individuals of Le(a+b+) and partial secretor phenotypes

  • Stephen M. Henry
  • Rafael Oriol
  • Bo E. Samuelsson
Non-Lectin Papers


Red cell Lewis antigens are carried by glycosphingolipids passively absorbed from plasma. Plasma was collected from a spectrum of individuals with normal and unusual Lewis/secretor phenotypes in order to investigate the glycolipid basis for the unusual phenotypes. Samples were obtained from: a Le(a+b−) ABH nonsecretor who secreted Lewis substances; a Le(a+b−) partial secretor; Le(a+b+) partial secretors; Le(a+b+) secretors; and a full range of normal Lewis/secretor phenotypes as controls. The Le(a+b+) samples represented Polynesian, Asian and Réunion Island ethnic backgrounds. Nonacid glycolipids were prepared, separated by thin-layer chromatography, and then immunostained with potent monoclonal antibodies of known specificity. Despite different serological profiles of the Le(a+b−) and Le(a+b+) Polynesian samples, their plasma glycolipid expressions were very similar, with both Lea and Leb co-expressed. The copresence of Lea and Leb in Le(a+b+) samples is in marked contrast to Caucasians with normal Lewis phenotypes, who have predominantly either Lea or Leb. These results suggest that there is a range of the secretor transferases in different individuals, possibly due to different penetrance or to several weak variants. We also show that Lewis epitopes on longer and/or more complex core chains appear to be predominant in the Polynesian Le(a+b+) samples. The formation of these extended glycolipids is compatible with the concept that in the presence of reduced secretor fucosyltransferase activity, increased elongation of the precursor chain occurs, which supports the postulate that fucosylation of the precursor prevents or at least markedly reduces chain elongation.


Lewis antigens glycolipids Le(a+b+) plasma secretor 



chromatogram binding assay


thin-layer chromatography


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  1. 1.
    Oriol R, Le Pendu J, Mollicone R (1986)Vox Sang 51: 161–71.Google Scholar
  2. 2.
    Clausen H, Hakomori S (1989)Vox Sang 56:1–20.Google Scholar
  3. 3.
    Mourant AE (1946)Nature 158:237–8.Google Scholar
  4. 4.
    Andresen PH (1948)Acta Path Microbiol Scand 25:728.Google Scholar
  5. 5.
    Marcus DM, Cass LE (1969)Science 164:553–55.Google Scholar
  6. 6.
    Oriol R, Danilovs J, Lemieux R, Terasaki P, Bernoco D (1980)Hum Immunol 3:195–205.Google Scholar
  7. 7.
    Dunstan RA, Marcus B, Simpson MD, Rosse WF (1985)Am J Clin Pathol 83:90–94.Google Scholar
  8. 8.
    Grubb R (1948)Nature 162:933.Google Scholar
  9. 9.
    Brendemoen OJ (1950)J Med Clin 36:335–41.Google Scholar
  10. 10.
    Race RR, Sanger R (1975) InBlood Groups in Man, 6th ed.: pp. 311–22. Oxford: Blackwell Scientific Publications.Google Scholar
  11. 11.
    Henry SM, Simpson LA, Woodfield DG (1988)Hum Hered 38:111–16.Google Scholar
  12. 12.
    Henry SM, Simpson LA, Benny AG, Woodfield DG (1989)NZ J Med Lab Technol 43:64–67.Google Scholar
  13. 13.
    Henry SM, Dent AM, Harding Y (1990) InProceedings of the second international workshop and symposium on monoclonal antibodies against human red blood cells and related antigens. Lund, p. 75.Google Scholar
  14. 14.
    Henry SM, Benny AG, Woodfield DG (1990)Vox Sang 58:61–66.Google Scholar
  15. 15.
    Henry SM, Woodfield DG, Samuelsson BE, Oriol R (1993)Vox Sang 65:62–69.Google Scholar
  16. 16.
    Vos GH, Comley P (1967)Acta Genet 17:495–510.Google Scholar
  17. 17.
    Sturgeon P, Arcilla MB (1970)Vox Sang 18:301–22.Google Scholar
  18. 18.
    Lin-Chu M, Broadberry RE, Chang FJ (1988)Transfusion 28:350–52.Google Scholar
  19. 19.
    Ventura M, Gibaud A, Le Pendu J, Hillaire D, Gérard G, Vitrac D, Oriol R (1988)Hum Hered 38:36–43.Google Scholar
  20. 20.
    Karlsson KA (1987)Methods Enzymol 138:212–20.Google Scholar
  21. 21.
    Magnani JL, Smith DF, Ginsburg V (1981)Anal Biochem 109:399–402.Google Scholar
  22. 22.
    Hansson GC, Karlsson KA, Larson G, Samuelsson BE, Thurin J, Bjursten LM (1985)J Immunol Methods 83:37–42.Google Scholar
  23. 23.
    Good AH, Yau O, Lamontagne LR, Oriol R (1992)Vox Sang 62:180–89.Google Scholar
  24. 24.
    Henry SM, Oriol R, Samuelsson BE (1994)Vox Sang (in press).Google Scholar
  25. 25.
    Mourant AE, Kopec A, Domaniewska-Sobczak K (1976) InThe Distribution of the Human Blood Groups and Other Polymorphisms, 2nd ed.: pp. 548–76. Oxford: Oxford University Press.Google Scholar
  26. 26.
    Lin-Chu M, Broadberry RE (1990) InProceedings of the second international workshop and symposium on monoclonal antibodies against human red blood cells and related antigens. Lund: p. 79.Google Scholar
  27. 27.
    Henry SM, Samuelsson B, Oriol R (1994)Glycoconjugate J 11:600–7.Google Scholar
  28. 28.
    Stroud MR, Levery SB, Nudelman ED, Salyan MEK, Towell JA, Roberts CE, Watanabe M, Hakomori S (1991)J Biol Chem 266:8439–46.Google Scholar
  29. 29.
    Stroud MR, Levery SB, Salyan MEK, Roberts CE, Hakomori S (1992)Eur J Biochem 203:577–86.Google Scholar
  30. 30.
    Watanabe M, Ohishi T, Kuzuoka M, Nudelman ED, Stroud MR, Kubota T, Kodaira S, Abe O, Hirohashi S, Shimosata Y, Hakomori S (1991)Cancer Res 51:2199–204.Google Scholar

Copyright information

© Chapman & Hall 1994

Authors and Affiliations

  • Stephen M. Henry
    • 1
    • 2
  • Rafael Oriol
    • 3
  • Bo E. Samuelsson
    • 1
  1. 1.Department of Clinical Chemistry and Transfusion MedicineUniversity of GöteborgSweden
  2. 2.Department of Transfusion MedicineAuckland Regional Blood CentreAucklandNew Zealand
  3. 3.INSERM U178Villejuif 94807 CedexFrance

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