Glycoconjugate Journal

, Volume 11, Issue 5, pp 462–468 | Cite as

Expression of galectins on microvessel endothelial cells and their involvement in tumour cell adhesion

  • Reuben Lotan
  • Paula N. Belloni
  • Robert J. Tressler
  • Dafna Lotan
  • Xiao-Chun Xu
  • Garth L. Nicolson
Lectin Papers

Abstract

Lactoside-binding lectins (galectins) with molecular weights of about 14.5 kDa (galectin-1) and 29–35 kDa (galectin-3) bind preferentially to polylactosaminoglycan-containing glycoconjugates and have been found on the surface of tumour cells and implicated in cell-cell and cell-extracellular matrix adhesion and metastasis. We have demonstrated by immunoblotting that both galectin-1 and galectin-3 are present in extracts of endothelial cells cultured from bovine aorta, rat lung, mouse lung and mouse brain microvessels, whereas mouse hepatic sinusoidal endothelial cells expressed primarily galectin-1. These galectins were also localized by indirect immunofluorescent labelling on the surface of the different endothelial cells in culture and by immunohistochemical staining in human tissuesin vivo. Anti-galectin-1 antibodies inhibited the adhesion of liver-preferring murine RAW117-H10 large-cell lymphoma cells to hepatic sinusoidal endothelial cells or lung microvessel endothelial cellsin vitro. The data indicate that galectin-1 is expressed on the extracellular surface of endothelial cells and can mediate in part the adhesion of RAW117-H10 cells to liver microvessel endothelial cells.

Keywords

galectin lectin cell adhesion endothelial cell metastasis 

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Copyright information

© Chapman & Hall 1994

Authors and Affiliations

  • Reuben Lotan
    • 1
  • Paula N. Belloni
    • 1
  • Robert J. Tressler
    • 1
  • Dafna Lotan
    • 1
  • Xiao-Chun Xu
    • 1
  • Garth L. Nicolson
    • 1
  1. 1.Department of Tumor BiologyThe University of Texas, M. D. Anderson Cancer CenterUSA
  2. 2.SyntexPalo AltoUSA
  3. 3.GlycomedAlamedaUSA

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