Glycoconjugate Journal

, Volume 10, Issue 1, pp 120–126 | Cite as

Different binding capacities of influenza A and Sendai viruses to gangliosides from human granulocytes

  • Johannes Müthing
  • Frank Unland
  • Dagmar Heitmann
  • Michaela Orlich
  • Franz-Georg Hanisch
  • Jasna Peter-Katalinić
  • Vera Knäuper
  • Harald Tschesche
  • Sørge Kelm
  • Roland Schauer
  • Jürgen Lehmann
Papers

Abstract

The structures of gangliosides from human granulocytes were elucidated by fast atom bombardment mass spectrometry and by gas chromatography/mass spectrometry as their partially methylated alditol acetates. In human granulocytes besides GM3 (II3Neu5Ac-LacCer), neolacto-series gangliosides (IV3Neu5Ac-nLcOse4Cer, IV6Neu5Ac-nLcOse4Cer and VI3Neu5Ac-nLcOse6Cer) containing C24:1, and to some extent C22:0; and C16:0 fatty acid in their respective ceramide portions, were identified as major components. In this study we demonstrate that gangliosides from human granulocytes, the second most abundant cells in peripheral blood, can serve as receptors for influenza viruses A/PR/8/34 (H1N1), A/X-31 (H3N2), and a parainfluenza virus Sendai virus (HNF1, Z-strain). Viruses were found to exhibit specific adhesion to terminal Neu5Acα2-3Gal and/or Neu5Acα2-6Gal sequences as well as depending on the chain length of ganglioside carbohydrate backbones from human granulocytes, these important effector cells which represent the first line of defence in immunologically mediated reactions.

Keywords

Gangliosides human granulocytes TLC overlay assay receptor influenza A virus Sendai virus 

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Copyright information

© Chapman & Hall 1993

Authors and Affiliations

  • Johannes Müthing
    • 1
  • Frank Unland
    • 1
  • Dagmar Heitmann
    • 1
  • Michaela Orlich
    • 2
  • Franz-Georg Hanisch
    • 3
  • Jasna Peter-Katalinić
    • 4
  • Vera Knäuper
    • 5
  • Harald Tschesche
    • 5
  • Sørge Kelm
    • 6
  • Roland Schauer
    • 6
  • Jürgen Lehmann
    • 1
  1. 1.Institut für ZellkulturtechnikUniversität BielefeldBielefeldGermany
  2. 2.Institut für VirologieJustus-Liebig-Universität GießenGießenGermany
  3. 3.Institut für ImmunbiologieUniversität KölnKöln 15Germany
  4. 4.Institut für Physiologische ChemieUniversität BonnBonnGermany
  5. 5.Institut für BiochemieUniversität BielefeldBielefeldGermany
  6. 6.Institut für BiochemieChristian-Albrechts Universität KielKielGermany

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