Glycoconjugate Journal

, Volume 9, Issue 1, pp 27–38 | Cite as

Production and characterization of a monoclonal antibody (BBH5) directed to ganglioside lactone

  • Bernadette Bouchon
  • Steven B. Levery
  • Henrik Clausen
  • Sen-Itiroh Hakomori
Papers
Received:
Revised:

Abstract

The occurrence of lactones in various ganglioside preparations has been clearly demonstrated, yet the natural occurrence of ganglioside lactones in cells and tissues has been the subject of long debate, since lactones can be formed readily during preparation of gangliosides. We now report the generation of a monoclonal antibody (BBH5) that reacts specifically with lactones of disialogangliosides having the NeuAc2-8NeuAc2-3Gal sequence, but does not crossreact with the parent ganglioside. The specificity of the antibody resides on the first lactone ring between two sialic acid residues but not on the second lactone ring between sialic acid and galactose, as evidenced by reactivity with lactonized GD1b having the first lactone ring (L1), and by reactivity with lactonized polysialic acid homo-oligomers ([NeuAcα2-8] n NeuAc). The sialic acid carboxyl involved in the lactone ring was unequivocally determined after ammonolysis followed by methylation and fast atom bombardment mass spectrometry. The antibody BBH5 thus provides a novel tool for studies of the natural occurrence of lactones in cells and tissues.

Keywords

Lactone ganglioside monoclonal antibody sialic acid 

Abbreviations

BSA

bovine serum albumin

CM

chloroform-methanol

CMW

chloroform-methanol-water

FAB-MS

fast atom bombardment mass spectrometry

IHW

isopropanol-hexane-water

MAb

monoclonal antibody

PBS

phosphate-buffered saline

TLC

thin layer chromatography

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Copyright information

© Chapman and Hall 1992

Authors and Affiliations

  • Bernadette Bouchon
    • 1
    • 2
  • Steven B. Levery
    • 1
    • 2
  • Henrik Clausen
    • 1
    • 2
  • Sen-Itiroh Hakomori
    • 1
    • 2
  1. 1.The Biomembrane InstituteSeattleUSA
  2. 2.University of WashingtonSeattleUSA

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